MAB331-C

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free)

clone SP14, from mouse

• 同義詞: Synaptosomal-associated protein 25, SNAP-25, Super protein, SUP, Synaptosomal-associated 25 kDa protein, SNAP-25
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: SP14, monoclonal
• 物種活性: monkey, bovine, mouse, rat, guinea pig, human
• 技術: activity assay: suitable; electron microscopy: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: NP_570824
• UniProt登錄號: P60880
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SNAP25(6616)

描述

一般說明

Synaptosomal-associated protein 25 (UniProt P60880; also known as SNAP-25, SUP, Super protein, Synaptosomal-associated 25 kDa protein) is encoded by the SNAP25 (also known as SNAP) gene (Gene ID 6616) in human. SNAP-25 belongs to the superfamily of soluble N-ethylmaleimide-sensitive factor (NSF) attachment protein (SNAP) receptor (SNARE) proteins involved in mediating vesicle fusion events. Target or t-SNAREs located in the membranes of target compartments form stable subcomplexes and engage vesicle or v-SNAREs in the membranes of transport vesicles to facilitate the fusion. SNAP-25 is a t-SNARE protein of the trans-SNARE complex that executes fusion between the synaptic vesicle and plasma membrane. SANP-25 does not have a transmembrane domain and is anchored to the cytosolic face of membranes via palmitoyl side chains covalently linked to cysteine residues.

特異性

Clone SP41 recognizes both SNAP-25 isoforms A & B. Clone SP41 is reactive toward endogenous or exogenously expressed SNAP-25 in COS cells, but not SNAP-25 expressed in E. coli, indicative of a posttranslational modification-dependent antigenicity.

免疫原

Epitope: N-terminal half

Human brain proteins immunoprecipitated with EP10 (Cat. No. MAB332).

應用

Anti-SNAP-25 Antibody, clone SP14 (Ascites Free) is an antibody against SNAP-25 for use in Western Blotting, Immunohistochemistry (Paraffin), Electron Microscopy and Activity Assay.

Research Category
Neuroscience

Research Sub Category
Developmental Neuroscience

品質

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected SNAP-25 in 10 µg of human brain tissue lysate.

標靶描述

~25 kDa observed

聯結

Replaces: MAB331

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl without preservatives.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable