MAB3140

Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9

clone 4E9, Chemicon^®, from mouse

• 同義詞: NHE1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: 4E9, monoclonal
• 物種活性: amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)
• 物種活性（以同源性預測）: mouse, mammals, rat
• 製造商／商標名: Chemicon^®
• 技術: western blot: suitable
• 同型: IgG1
• 適合性: not suitable for immunoprecipitation
• NCBI登錄號: NM_003047.2
• UniProt登錄號: P19634
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... SLC9A1(6548)

描述

一般說明

Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.

特異性

Na+/H+ exchanger, isoform NHE1

免疫原

Epitope: C-terminus

MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.

應用

Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.

Immunoblotting:
1:1,000 dilution used on a previous lot.

Optimal working dilutions must be determined by the user.

品質

Routinely evaluated by Western Blot on Human Kidney lysates.

Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.

標靶描述

~100-110 kDa

外觀

Protein A purified

Format: Purified

Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide

儲存和穩定性

Stable for 1 year at 2-8ºC from date of receipt.

分析報告

Control
Kidney tissue (basolateral membrane)

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable