推薦產品
生物源
mouse
品質等級
抗體表格
ascites fluid
抗體產品種類
primary antibodies
無性繁殖
1C3, monoclonal
物種活性
mouse, human, rat
製造商／商標名
Chemicon®
技術
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
同型
IgG1κ
適合性
not suitable for flow cytometry
not suitable for immunoprecipitation
NCBI登錄號
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... FRAXA(2477) , FRAXA(108684022)
一般說明
Fragile X Mental Retardation Protein (FMRP) is a RNA-binding protein that is associated to polysomes and may be involved in the transport of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplification of a CGG repeat region which is directly in front of the coding region.
特異性
Human and mouse FMRP. The epitope is localized in the N-terminal half of FMRP. Cross reaction with FXR protein may be detected in cases of high expression of the latter proteins.
免疫原
Fusion protein with a full length FMRP (human).
應用
Anti-Fragile X Mental Retardation Protein Antibody, clone 1C3 detects level of Fragile X Mental Retardation Protein & has been published & validated for use in ELISA, IC, IH, IH(P) & WB.
Immunohistochemistry:
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)
Optimal working dilutions must be determined by the end user.
Frozen and paraffin sections. 1:500-1:5,000 dilution of a previous lot was used.
ELISA:
A 1:500-1:5,000 dilution of a previous lot was used in ELISA.
Detection of FMRP on Blood Smears:
A 1:500-1:5,000 dilution of a previous lot was used.
Immunocytochemistry:
A 1:500-1:5,000 dilution of a previous lot was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% triton in block only)
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurodegenerative Diseases
RNA Binding Protein (RBP)
Neurodegenerative Diseases
RNA Binding Protein (RBP)
品質
Evaluated by Western Blot on Mouse E17 spinal cord lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.
Western Blotting Analysis:
1:500 dilution of this antibody detected Fragile X Mental Retardation Protein on 10 μg of Mouse E17 spinal cord lysates.
標靶描述
~71 kDa
外觀
Unpurified
Ascites mouse monoclonal IgG1k fluid containing no preservatives
儲存和穩定性
Stable for 1 year at -20ºC from date of receipt.
分析報告
Control
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Fragile X mental retardation protein targets G quartet mRNAs important for neuronal function.
J C Darnell, K B Jensen, P Jin, V Brown, S T Warren, R B Darnell
Cell null
The RNA-binding protein fragile X-related 1 regulates somite formation in Xenopus laevis.
Huot, ME; Bisson, N; Davidovic, L; Mazroui, R; Labelle, Y; Moss, T; Khandjian, EW
Molecular Biology of the Cell null
C H Lin et al.
Journal of biochemistry, 128(3), 493-498 (2000-08-31)
Arginine methylation in RNA-binding proteins containing arginine- and glycine-rich RGG motifs is catalyzed by specific protein arginine N-methyltransferase in cells. We previously showed that lymphoblastoid cells grown in the presence of an indirect methyltransferase inhibitor, adenosine dialdehyde (AdOx), accumulated high
Sally M Till et al.
Human molecular genetics, 24(21), 5977-5984 (2015-08-06)
Recent advances in techniques for manipulating genomes have allowed the generation of transgenic animals other than mice. These new models enable cross-mammalian comparison of neurological disease from core cellular pathophysiology to circuit and behavioural endophenotypes. Moreover they will enable us
Rapid antibody test for fragile X syndrome.
Willemsen, R, et al.
Lancet, 345, 1147-1148 (1995)
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