生物源
mouse
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
P2H3, monoclonal
物種活性
human
製造商／商標名
Chemicon®
技術
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
同型
IgG1
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... SELE(6401)
一般說明
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
特異性
Human E-Selectin
免疫原
IL-1(1 activated HUVEC (human)
應用
Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.
Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
外觀
Format: Purified
Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN3.
儲存和穩定性
Maintain at 2-8°C for up to 12 months from date of receipt..
分析報告
Control
Ductal breast cancer or mormal colon
Ductal breast cancer or mormal colon
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
H E Young et al.
The Anatomical record, 264(1), 51-62 (2001-08-16)
This study details the profile of 13 cell surface cluster differentiation markers on human reserve stem cells derived from connective tissues. Stem cells were isolated from the connective tissues of dermis and skeletal muscle derived from fetal, mature, and geriatric
Preparation of chitosan films using different neutralizing solutions to improve endothelial cell compatibility.
Qing He,Qiang Ao,Yandao Gong,Xiufang Zhang
Journal of Materials Science. Materials in Medicine null
Natalya V Narizhneva et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 19(9), 1158-1160 (2005-04-19)
Expression of cell adhesion molecules (CAM) responsible for leukocyte-endothelium interactions plays a crucial role in inflammation and atherogenesis. Up-regulation of vascular CAM-1 (VCAM-1), intracellular CAM-1 (ICAM-1), and E-selectin expression promotes monocyte recruitment to sites of injury and is considered to
Enhanced expression of alpha(1,3)-fucosyltransferase genes correlates with E-selectin-mediated adhesion and metastatic potential of human lung adenocarcinoma cells.
Martin-Satue, M, et al.
Cancer Research, 58, 1544-1550 (1998)
Preparation and characterization of chitosan-heparin composite matrices for blood contacting tissue engineering.
He Q, Ao Q, Gong K, Zhang L, Hu M, Gong Y, Zhang X
Biomedical Materials (Bristol, England) null
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