MAB1984-I
Anti-Integrin α5β1 Antibody, clone BMA5
culture supernatant, clone BMA5, from rat
同義詞:
Integrin alpha-5, CD49 antigen-like family member E, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5, CD49e, Integrin alpha-5 heavy chain, Integrin alpha-5 light chain, Integrin α5β1, Integrin beta-1, Fibronectin receptor subunit be
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About This Item
分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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一般說明
Integrin alpha-5 (UniProt P11688; CD49 antigen-like family member E, CD49e, Fibronectin receptor subunit alpha, Integrin alpha-F, VLA-5) and Integrin beta-1 (UniProt P09055; CD29, Fibronectin receptor subunit beta, GpIIa, VLA-4 subunit beta) are encoded by the Itga5 (VLA5, Fnra; Gene ID 16402) and the Itgb1 (Fnrb; Gene ID 16412) gene, respectively, in murine species. The integrin family of cell adhesion receptors consists of at least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based upon the pairing abilities of the individual alpha and beta subunits. Key to these molecular interactions between the integrin receptors and their respective ligands is the recognition of the Arg-Gly-Asp (RGD) sequence, known to be present in the extracellular matrix components fibronectin, vitronectin, collagen, fibrinogen, and von Willebrand factor. The involvement of integrins in vascular proliferation, adhesion, and wound repair has been well documented. The adhesion receptor alphaVbeta1 is also known as VLA-5 (very late antigen-5) and the primary receptor for fibronectin. VLA-5 is reported to be involved many important physiological events, including angiogenesis, inflammation, neural crest development and cardiovascular morphogenesis.
免疫原
SSC-VII cells.
應用
Immunoprecipitation Analysis: A representative lot immunoprecipitated integrin VLA-5 (αVβ1) complex from murine cells, including SSC-VII oral cavity cancer cells, B16F1 melanoma cells, and mast cells (CFTL-15 & MC/9, BMMCs) (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
Affects Function: A representative lot suppressed β2GPI surface expression on RAW264.7 cells and attenuated cardiolipin-β2GPI (aCL/β2GPI) complex- and gelsolin-mediated cellular p38 and FAK phosphorylation (Bohgaki, M., et al. (2011). J. Cell. Mol. Med. 15(1):141-151).
Affects Function: A representative lot, when combined with anti-Integrin β3 antibody, greatly blocked EphA8-stimulated NIH/3T3 adhesion to fibronectin surface (Gu, C., and Park, S. (2001). Mol Cell Biol. 21(14):4579-4597).
Affects Function: A representative lot, when combined with anti-Integrin α4, clone PS/2 (Cat. No. CBL1304), greatly blocked PMA-stimulated adhesion of spleen T-cells from non-obese diabetic (NOD) mice to fibronectin-coated surface (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Affects Function: A representative lot, when administered together with anti-Integrin α4, clone PS/2 (Cat. No. CBL1304) via intraperitoneal injection, greatly reduced pancreas islet leukocyte infiltration among non-obese diabetic (NOD) mice following diabetes onset induction by cyclophosphamide injection (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Affects Function: A representative lot blocked integrin αVβ1-mediated adhesion of murine bone marrow-derived mast cells (BMMCs) to fibronectin-coated surface upon PMA stimulation (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of murine peritoneal macrophages and peripheral blood monocytes (Kapetanovic, R., et al. (2011). Am. J. Physiol. Cell Physiol. 300(4):C850-859).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of murine embryonic fibroblasts (Gupta, S.K., and Vlahakis, N.E. (2009). J. Cell Sci. 122(Pt 12):2043-2054).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of CD4+ or CD8+ T-cells from peripheral blood and spleen of non-obese diabetic (NOD) mice (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of murine bone marrow-derived mast cells (BMMCs) (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
Affects Function: A representative lot suppressed β2GPI surface expression on RAW264.7 cells and attenuated cardiolipin-β2GPI (aCL/β2GPI) complex- and gelsolin-mediated cellular p38 and FAK phosphorylation (Bohgaki, M., et al. (2011). J. Cell. Mol. Med. 15(1):141-151).
Affects Function: A representative lot, when combined with anti-Integrin β3 antibody, greatly blocked EphA8-stimulated NIH/3T3 adhesion to fibronectin surface (Gu, C., and Park, S. (2001). Mol Cell Biol. 21(14):4579-4597).
Affects Function: A representative lot, when combined with anti-Integrin α4, clone PS/2 (Cat. No. CBL1304), greatly blocked PMA-stimulated adhesion of spleen T-cells from non-obese diabetic (NOD) mice to fibronectin-coated surface (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Affects Function: A representative lot, when administered together with anti-Integrin α4, clone PS/2 (Cat. No. CBL1304) via intraperitoneal injection, greatly reduced pancreas islet leukocyte infiltration among non-obese diabetic (NOD) mice following diabetes onset induction by cyclophosphamide injection (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Affects Function: A representative lot blocked integrin αVβ1-mediated adhesion of murine bone marrow-derived mast cells (BMMCs) to fibronectin-coated surface upon PMA stimulation (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of murine peritoneal macrophages and peripheral blood monocytes (Kapetanovic, R., et al. (2011). Am. J. Physiol. Cell Physiol. 300(4):C850-859).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of murine embryonic fibroblasts (Gupta, S.K., and Vlahakis, N.E. (2009). J. Cell Sci. 122(Pt 12):2043-2054).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of CD4+ or CD8+ T-cells from peripheral blood and spleen of non-obese diabetic (NOD) mice (Uniyal, S., et al. (1999). J. Autoimmun. 12(3):167-176).
Flow Cytometry Analysis: A representative lot detected integrin VLA-5 (αVβ1) immunoreactivity on the surface of murine bone marrow-derived mast cells (BMMCs) (Fehlner-Gardiner, C.C., et al. (1996). Differentiation. 60(5):317-325).
This Anti-Integrin α5β1 Antibody, clone BMA5 is validated for use in Flow Cytometry, Immunoprecipitation, Affects Function for the detection of Integrin α5β1.
品質
Evaluated by Flow Cytometry in NIH/3T3 cells.
Flow Cytometry Analysis: 100 µL of 1:200 dilution from this antibody detected Integrin α5β1 in NIH/3T3 cells.
Flow Cytometry Analysis: 100 µL of 1:200 dilution from this antibody detected Integrin α5β1 in NIH/3T3 cells.
標靶描述
115 kDa calculated
聯結
Replaces: MAB1984
外觀
Rat monoclonal IgG2bκ antibody in tissue culture supernatant without preservatives.
其他說明
Concentration: Please refer to lot specific datasheet.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 2
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Miyuki Bohgaki et al.
Journal of cellular and molecular medicine, 15(1), 141-151 (2009-10-21)
Antiphospholipid syndrome (APS) is characterized by thrombosis and the presence of antiphospholipid antibodies (aPL) that directly recognizes plasma β(2)-glycoprotein I (β(2) GPI). Tissue factor (TF), the major initiator of the extrinsic coagulation system, is induced on monocytes by aPL in
Differential utilization of VLA-4 (alpha 4 beta 1) and -5 (alpha 5 beta 1) integrins during the development of mouse bone marrow-derived mast cells.
Fehlner-Gardiner, CC; Uniyal, S; von Ballestrem, CG; Chan, BM
Differentiation null
Leukocytes utilize both alpha4 and alpha5 integrins for intraislet infiltration in non-obese diabetic mice.
Uniyal, S; Boeters, L; Chakrabarti, S; Singh, B; Chan, BM
Journal of Autoimmunity null
Mechanisms of TNF induction by heat-killed Staphylococcus aureus differ upon the origin of mononuclear phagocytes.
Kapetanovic, R; Parlato, M; Fitting, C; Quesniaux, V; Cavaillon, JM; Adib-Conquy, M
American Journal of Physiology. Cell Physiology null
Integrin alpha9beta1 mediates enhanced cell migration through nitric oxide synthase activity regulated by Src tyrosine kinase.
Gupta, SK; Vlahakis, NE
Journal of Cell Science null
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