MAB1944-C

Anti-Collagen VI alpha-3 Antibody, clone 3C4, Ascites Free

culture supernatant, clone 3C4, from mouse

• 同義詞: Collagen alpha-3(VI) chain, Collagen VI alpha-3 polypeptide
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: culture supernatant
• 抗體產品種類: primary antibodies
• 無性繁殖: 3C4, monoclonal
• 物種活性: human
• 技術: electron microscopy: suitable; flow cytometry: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable
• 同型: IgG1κ
• NCBI登錄號: NP_004360
• UniProt登錄號: P12111
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... COL6A3(1293)

描述

一般說明

Collagen alpha-3(VI) chain (UniProt P12111; also known as Collagen VI alpha-3 polypeptide) is encoded by the COL6A3 gene (Gene ID 1293) in human. Type VI collagen is an extracellular matrix (ECM) component present in virtually all connective tissues, including cartilage, bone, tendon, muscles and cornea, where it forms microfibrils in close association with basement membranes. In addition to anchoring the basement membrane to the pericellular matrix in muscle, research also indicates a role for collagen VI in cell signaling and cell migration. The basic structural unit of collagen VI is a heterotrimer composed of the alpha-1(VI), alpha-2(VI), and alpha-3(VI) chains (encoded by the COL6A1, COL6A2, and COL6A3 genes, respectively). The α1(VI) and α2(VI) chains are similar in size and domain structure, they contain a 335- or 336-amino acid triple helix region that is characteristic of all collagens. Flanking the triple helix are domains homologous to the A-type domains found in von Willebrand factor (VWA domains). α1(VI) and α2(VI) contain one VWA domain N-terminal to the triple helix (N1) and two VWA domains C-terminal of the helix (C1 and C2). The α3(VI) chain, on the other hand, is much larger with 10 N-terminal (N1–N10) and two C-terminal VWA domains (C1 and C2), and several other types of identifiable domains in the C terminal region (C3–C5). Mutations in the COL6A1, COL6A2, and COL6A3 genes are known causes of Ullrich congenital muscular dystrophy (UCMD) and Bethlem myopathy (BM). Three additional type VI collagen chains have been reported in 2008 (α4(VI), α5(VI) and α6(VI) chains encoded by COL6A4, COL6A5, and COL6A6, respectively).

免疫原

Purified human collagen VI.

應用

This Anti-Collagen VI alpha-3 Antibody, clone 3C4, Ascites Free is validated for use in Immunohistochemistry (Paraffin), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Electron Microscopy for the detection of Collagen VI alpha-3.

品質

Evaluated by Immunohistochemistry in human breast tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected collagen VI alpha-3 chain immunoreactivity in human breast tissue section.

標靶描述

343.7/321.4/113.2/278.2/134.7 kDa (isoform 1/2/3/4/5 pro-form) and 340.8/318.5/110.4/275.3/131.8 kDa (isoform 1/2/3/4/5 mature form) calculated

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2