MAB13431

Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2

clone A-Gel VC2, Chemicon^®, from mouse

• 同義詞: Gelatinase A, 72 kDa Type IV Collagenase
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: A-Gel VC2, monoclonal
• 物種活性: human
• 製造商／商標名: Chemicon^®
• 技術: ELISA: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
• 同型: IgG1
• 適合性: not suitable for Western blot
• NCBI登錄號: NM_004530.2
• UniProt登錄號: P08253
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MMP2(4313)

描述

一般說明

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

特異性

MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic.

免疫原

Epitope: pro and active form

Human APMA-activated native 72kDa Gelatinase A.

應用

Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).

Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

ELISA (not suitable for coating, contains BSA) 1:200-1:2000

Not suitable for Western blotting


Optimal working dilution should be determined by the end user.

Research Category
Cell Structure

Research Sub Category
MMPs & TIMPs

聯結

Replaces: 04-1048

外觀

10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide

Format: Purified

儲存和穩定性

Antibody is stable for 12 months when stored at 2-8ºC

分析報告

Control
POSTIVE CONTROL:

Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable