MAB1271-I

Anti-Golgi Zone Antibody, clone 371-4

• 分類程式碼代碼: 12352203
• NACRES: NA.74
• 共軛: unconjugated
• application: ICC
• 無性繁殖: 371-4, monoclonal
• 物種活性: human
• 技術: immunocytochemistry: suitable

屬性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: 371-4, monoclonal
• 分子量: calculated mol wt 134.55 kDa
• 純化經由: using protein G
• 物種活性: human
• 物種活性（以同源性預測）: monkey
• 包裝: antibody small pack of 100 μg
• 技術: immunocytochemistry: suitable
• 同型: IgG1κ
• 表位序列: Unknown
• Protein ID登錄號: NP_001139138
• UniProt登錄號: Q92896
• 運輸包裝: 2-8°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GLG1(2734)

描述

一般說明

Golgi apparatus protein 1 (UniProt: Q92896; also known as CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand 1, ESL-1, Golgi sialoglycoprotein MG-160) is encoded by the GLG1 (also known as CFR1, ESL1, MG160) gene (Gene ID: 2734) in human. The Golgi apparatus plays a major role in transport, processing, and sorting of thousands of macromolecules in eukaryotic cells. It is readily recognizable because of its stacked membrane-coated cavities known as dictyosomes. The Golgi complex consists of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face and each of these regions contain various enzymes to selectively modify the macromolecules that pass through this complex. The Golgi complex is localized in the vicinity of the nucleus and is usually very large in secretory cells, where it fills most of the cytoplasm. Clone 371-4 selectively recognizes the Golgi complex in human cells. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells.

特異性

Clone 371-4 is a mouse monoclonal antibody that detects Golgi apparatus.

免疫原

SU-DHL-1 human large cell lymphoma cells.

應用

Quality Control Testing

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in HeLa cells.

Tested Application

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Golgi apparatus protein 1 in A431cells.

Immunocytochemistry Analysis: A representative lot detected Golgi Zone in Immunocytochemistry applications (Endo, Y., et al. (2007). Cancer Sci.;98(10):1633-7; Strehlow, A.N., et al. (2007). Hum Mol Genet.;16(4):391-409; Oiso, S., et al. (2009). J Neurochem.;109(1):158-67; Leite, C., et al. (2014). PLoS One.;9(10):e111059).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Golgi Zone, clone 371-4, Cat. No. MAB1271-I, is a mouse monoclonal antibody that detects Golgi apparatus protein 1 and is tested for use in Immunocytochemistry.

外觀

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable