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Merck
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文件

M04S-03-5PK

Millipore

CellASIC ONIX切换板哺乳动物细胞（4腔室）

The M04 switching plates enable continuous perfusion culture for live cell analysis. Laminar flow provides rapid & uniform solution exchange for maximum cell health. The glass coverslide bottom surface ensures the highest resolution optical viewing.

同義詞:

CellASIC ONIX Plate, Mammalian Cell Plate, ONIX Switching Plate

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About This Item

分類程式碼代碼:
41104923
eCl@ss:
32011202
NACRES:
NB.22

材料

PMMA
glass
polycarbonate
polydimethylsiloxane (PDMS)

製造商／商標名

CellASIC® ONIX

寬度 × 長度 × 高度

85.48 mm × 127.76 mm × 14.35 mm

相容性

for use with cells sized up to 50.0 μm

運輸包裝

ambient

相關類別

一般說明

CellASIC® ONIX 哺乳细胞切换板（4 腔室）可实现连续灌注培养以进行活细胞分析。层流为最大限度细胞健康提供快速均匀的溶液交换。玻璃盖玻片底面可确保最高分辨率的光学观察。

應用

CellASIC® ONIX 哺乳细胞切换板（4 腔室）已用于：
  • 活细胞成像以研究phi-particle对动力蛋白的自动抑制以及动力蛋白激活蛋白活化动力蛋白的贡献。
  • 通过荧光显微镜技术进行fcSAgA结合和免疫球蛋白M表面聚集的活细胞成像
  • 多重表皮生长因子（EGF）脉冲实验
CellASIC® ONIX 转换板适用于：
  • 粘附细胞的延时分析（time-lapse analysis）
  • 长期连续灌注实验（通常3天）
  • 溶液交换实验（诱导、抑制、给药）
  • 培养室内活细胞的自动免疫染色和按需固定
  • 平行比较四种不同细胞类型或暴露条件（培养基成分）、温度和气体气氛控制（温度变化、缺氧条件等）

特點和優勢

  • 兼容CellASIC® ONIX2 微流体系统和ONIX2 Manifolds
  • 可以用溶液切换进行基于灌注的长期活细胞分析
  • 板为2D和3D细胞培养物提供受控的动态微环境。

法律資訊

CELLASIC is a registered trademark of Merck KGaA, Darmstadt, Germany

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

nwg

閃點（°F）

Not applicable

閃點（°C）

Not applicable

分析證明 (COA)

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Brittany L Hartwell et al.
Biomacromolecules, 18(6), 1893-1907 (2017-05-06)
A pressing need exists for antigen-specific immunotherapies (ASIT) that induce selective tolerance in autoimmune disease while avoiding deleterious global immunosuppression. Multivalent soluble antigen arrays (SAgAPLP:LABL), consisting of a hyaluronic acid (HA) linear polymer backbone cografted with multiple copies of autoantigen
Kai Zhang et al.
Cell, 169(7), 1303-1314 (2017-06-13)
Cytoplasmic dynein-1 binds dynactin and cargo adaptor proteins to form a transport machine capable of long-distance processive movement along microtubules. However, it is unclear why dynein-1 moves poorly on its own or how it is activated by dynactin. Here, we
Angel Stanoev et al.
Cell systems, 7(3), 295-309 (2018-08-27)
The proto-oncogenic epidermal growth factor receptor (EGFR) is a tyrosine kinase whose sensitivity to growth factors and signal duration determines cellular behavior. We resolve how EGFR's response to epidermal growth factor (EGF) originates from dynamically established recursive interactions with spatially

文章

Real-time imaging of adherent and non-adherent cell interactions: utility of an automated microfluidic trap platform to recapitulate in vivo cell culture microenvironment

This page describes novel microfluidic approaches to trap two different cell types in precise locations in order to image and study their interactions over time.

Primary neuron culture and analysis facilitated by the CellASIC® ONIX Microfluidic Platform

This page describes a novel, optimized microfluidic platform for long-term culture of primary neurons to monitor dynamic cellular processes in real time.

Primary Neuron Culture and Analysis Facilitated by the CellASIC® ONIX Microfluidic Platform

This page describes a novel, optimized microfluidic platform for long-term culture of primary neurons to monitor dynamic cellular processes in real time.

Dynamic Live Cell Imaging of Caspase Mediated Apoptosis and Cellular Hypoxia in Lymphocytes and Cancer Cells Using the CellASIC® ONIX2 Microfluidic Platform

Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.

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