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HTS158M

Sigma-Aldrich

ChemiSCREEN Membrane Preparation Recombinant Human α1B Adrenergic Receptor

Human alpha1B GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

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About This Item

分類程式碼代碼:
41106514
eCl@ss:
32161000
NACRES:
NA.41

生物源

human

品質等級

重組細胞

expressed in Chem-1 cells

製造商/商標名

ChemiScreen
Chemicon®

技術

ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

一般說明

Full-length human ADRA1B cDNA encoding α1B adrenergic
The endogenous catecholamines epinephrine and norepinephrine have profound effects on smooth muscle activity, cardiac function, carbohydrate and fat metabolism, hormone secretion, neurotransmitter release, and central nervous system actions. These activities are mediated by GPCRs belonging to two subfamilies, the α- and β-adrenoceptors (Bylund et al., 1994). The three members of the α1 subclass of adrenoceptors, α1A, α1B and α1D, couple to Gq, and promote contraction of vascular and urinary tract smooth muscle, relaxation of intestinal smooth muscle, increased contractile force in the heart, and glycogenolysis and gluconeogenesis in the liver. The different subtypes have overlapping distributions and variably contribute to these effects depending on species and tissue. Overexpression of a constitutively active α1B mutant in the heart of transgenic mice resulted in cardiac hypertrophy with increased heart weight/body weight ratios. Analysis of α1B knock out mice has provided evidence that α1B is a mediator of blood pressure and aortic contractile responses induced by α1 agonists (Milano et al., 1994). The locomotor and rewarding effects of pysochostimulants and opiates were suppressed in mice lacking α1B-adrenergic receptors (Drouin et al. 2002). Millipore′s α1B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of α1B. The membrane preparations exhibit a Kd of 0.8 nM for [3H]-Prazosin. With 1 nM [3H]-Prazosin, 5μg/well α1B Membrane Prep typically yields greater than 5-fold signal-to-background ratio.

應用

Radioligand binding assay and GTPγS binding.

生化/生理作用

GPCR Class: A
Protein Target: alpha1B
Target Sub-Family: Adrenergic

品質

Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of α1B Receptor membrane prep.
5 µg/well
Signal:Background 11.8
Specific Binding (cpm) 1171

SPECIFICATIONS: 1 unit = 5 µg membrane preparation
Bmax for [3H]-Prazosin binding: 12.9 pmol/mg protein
Kd ffor [3H]-Prazosin binding: ~0.8 nM

規格

Inucbation Conditions
RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM Tris, pH 7.4. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Tris, pH 7.4, 10 mM MgCl2, 1 mM EDTA, filtered and stored at 4°C

Radioligand: [3H]-Prazosin. (Perkin Elmer # NET823)

Wash Buffer: 50 mM Tris, pH 7.4, 500mM NaCl . 0.1% BSA filtered and stored at 4°C.

One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 5-fold signal:background with 3H labeled Prazosin at 1nM

外觀

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes protein were adjusted to 1 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C

儲存和穩定性

Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Myocardial expression of a constitutively active alpha 1B-adrenergic receptor in transgenic mice induces cardiac hypertrophy.
Milano, C A, et al.
Proceedings of the National Academy of Sciences of the USA, 91, 10109-10113 (1994)

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