HT17MAG14PMX25BK

MILLIPLEX^® Human TH17 Magnetic Bead Panel Premixed - 25 Plex - Space Saver (Bulk) Packaging

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

屬性

• 品質等級: 200
• 物種活性: human
• 製造商／商標名: Milliplex^®
• assay range: standard curve range: 1-5000 pg/mL; (IL-10); standard curve range: 10-40,000 pg/mL; (IFNγ); standard curve range: 12-50,000 pg/mL; (IL-17A & IL-2); standard curve range: 24-100,000 pg/mL; (IL-17 & IL-4); standard curve range: 3-10,000 pg/mL; (IL-6 & TNFα); standard curve range: 336-1,500,000 pg/mL; (IL-23); standard curve range: 37-150,000 pg/mL; (IL-22 & TNFβ); standard curve range: 488-2,000,000 pg/mL; (IL-17E/IL-25); standard curve range: 49-200,000 pg/mL; (IL-28a & IL-31); standard curve range: 5-20,000 pg/mL; (MIP-3α, IL-1β, IL-12 (p70), IL-15, IL-21 & IL-33); standard curve range: 6-25,000 pg/mL; (IL-5); standard curve range: 61-250,000 pg/mL; (GM-CSF & IL-27); standard curve range: 7-30,000 pg/mL; (IL-13); standard curve range: 9-35,000 pg/mL; (IL-9)
• 技術: multiplexing: suitable
• 相容性: configured for Premixed
• 檢測方法: fluorometric (Luminex xMAP)
• 運輸包裝: wet ice

描述

一般說明

CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ combined with IL-23 and IL-1β in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22 and TNFα to promote tissue inflammation.

Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.

MILLIPLEX^® Human TH17 Premixed Bead Panel is a 25-plex kit to be used for the simultaneous quantification of the following analytes in serum, plasma or culture supernatant samples: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α and TNFβ.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
and quantitation.

Panel Type: Cytokines/Chemokines

特異性

Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

UPDATE >>
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

• Analytes: GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17F, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-27, IL-28A, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
• Recommended Sample type: Serum, plasma, and cell culture samples
• Recommended Sample dilution: Neat
• Assay Run Time: Overnight
• Research Category: Inflammation & Immunology

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

其他說明

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

法律資訊

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS08,GHS05,GHS07,GHS09
• 訊號詞: Danger
• 危險聲明: H302 + H312 + H332,H317,H318,H373,H411
• 防範說明: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危險分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• 標靶器官: Respiratory Tract
• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3