HCKP2-11K

MILLIPLEX^® Human Immuno-Oncology Checkpoint Protein Panel 2 - Immuno-Oncology Multiplex Assay

• 分類程式碼代碼: 12161503
• NACRES: NA.47

屬性

• 物種活性: human
• 製造商／商標名: Milliplex^®
• 技術: multiplexing: suitable
• 檢測方法: fluorometric (Luminex^® xMAP^®)
• 儲存溫度: 2-8°C

描述

一般說明

Immune checkpoint proteins are regulators of immune activation and play a key role in maintaining immune homeostasis, preventing autoimmunity, and are involved in cancer immune escape. These immune system molecules demonstrate the ability to act both as agonists (co-stimulatory checkpoint molecules) or antagonists (co-inhibitory checkpoint molecules) of immune response. Immune checkpoint inhibitor drugs targeting inhibitory immune checkpoint proteins, such as CTLA-4 and PD-L1, have demonstrated efficacy in cancer immunotherapy by improving the antitumor immune response and saving lives in the treatment of many malignancies. Immune checkpoint proteins may express on immune cells and/or tumor cells as activating receptors or inhibitory receptors. Many immune checkpoint proteins are also expressed as soluble forms in circulation and in the tumor and tumor microenvironment and are putative biomarkers for immunotherapy in various diseases including cancer.MILLIPLEX^® Human Immuno-Oncology Checkpoint Protein Panel 2 is a 31-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma and tissue culture samples: 4-1BBL/TNFSF9, 5′-NT/CD73 (5′-nucleotidase), APRIL (TNFSF13), Arginase-1, B7-H2/ICOSL (Inducible T cell costimulator ligand), B7-H3/CD276, B7-H4/VTCN1 (V-set domain-containing T-cell activation inhibitor 1), B7-H5/VISTA (V-set immunoregulatory receptor), B7-H6 (Natural killer cell cytotoxicity receptor 3 ligand), BAFF/BLyS (TNFSF13B), CD25/IL-2Rα (Interleukin-2 receptor alpha), CD30/TNFRSF8, CD40L (CD40 ligand), CD137/4-1BB (TNFRSF9), CD226/DNAM-1 (DNAX accessory molecule 1), E-Cadherin, FGL1/Hepassocin (Fibrinogen-like protein 1), Galectin-1 (Gal-1), Galectin-3 (Gal-3), Granulysin, Granzyme B, IDO1 (Indoleamine 2,3-dioxygenase 1), MICA (MHC class I polypeptide-related sequence A), MICB (MHC class I polypeptide-related sequence B), Nectin-2 (PVRL2, CD112), Nectin-4 (PVRL4), OX40/CD134 (TNFRSF4), Perforin, PVR/CD155 (Poliovirus receptor cell adhesion molecule), Siglec-7 (Sialic acid-binding Ig-like lectin 7, CD328), Siglec-9 (Sialic acid-binding Ig-like lectin 9, CD329).

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

The Human Immuno-Oncology Checkpoint Protein Panel 2 bead-based multiplex panel, using the Luminex^® xMAP technology, enables the simultaneous analysis of 31 immune checkpoint proteins in human serum, plasma and cell culture samples.This is an overnight incubation assay (16-18 hours at 2-8°C).A maximum of 25 μL per well 1:2 diluted serum or plasma can be used. Tissue culture or other media may be run neat or appropriately diluted in control medium. Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.CD40 Ligand;4-1BBL/TNFSF9;Arginase-1;B7-H2/ICOSL;B7-H3/CD276;5′-NT/CD73;B7-H4/VTCN1;APRIL/TNFSF13;B7-H5/VISTA;CD25/IL-2Rα;B7-H6;CD137/4-1BB;Granzyme B;CD226/DNAM-1;CD30/TNFRSF8;E-Cadherin;FGL1/Hepassocin;Galectin-1 (GAL1);Galectin-3;Granulysin;IDO1;MICA;MICB;Nectin-2;BAFF/Blys;Nectin-4;OX40/CD134;PVR/CD155;Siglec-7;Siglec-9;Perforin

特點和優勢

Design your multiplex kit by choosing available analytes within this panel.

包裝

96-well plate

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

法律資訊

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS08,GHS05,GHS07,GHS09
• 訊號詞: Danger
• 危險聲明: H302 + H312 + H332,H317,H318,H373,H411
• 防範說明: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危險分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• 標靶器官: Respiratory Tract
• 儲存類別代碼: 10 - Combustible liquids