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HADK2MAG-61K

Millipore

MILLIPLEX® Human Adipokine Magnetic Bead Panel 2 - Endocrine Multiplex Assay

The Human Adipokine Magnetic Bead Panel 2, using the Luminex xMAP technology, enables the simultaneous analysis of nine protein biomarkers in human serum and plasma samples.

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About This Item

分類程式碼代碼:
12161503
eCl@ss:
32161000
NACRES:
NA.47

品質等級

物種活性

human

製造商/商標名

Milliplex®

assay range

accuracy: 87%
(IL-8)

accuracy: 91%
(TNFα)

accuracy: 94%
(Insulin)

standard curve range: 0.64-10,000 pg/mL
(IL-8)

standard curve range: 0.64-10,000 pg/mL
(NGF)

standard curve range: 0.64-10,000 pg/mL
(TNF-α)

standard curve range: 0.96-15,000 pg/mL
(IL-6)

standard curve range: 1.3-20,000 pg/mL
(IL-1β)

standard curve range: 1.3-20,000 pg/mL
(MCP-1)

standard curve range: 38-600,000 pg/mL
(Leptin)

standard curve range: 9.6-150,000 pg/mL
(HGF)

standard curve range: 9.6-150,000 pg/mL
(Insulin)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-1β)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-6)

inter-assay cv: <15%
intra-assay cv: <10%
(IL-8)

inter-assay cv: <15%
intra-assay cv: <10%
(Insulin)

inter-assay cv: <15%
intra-assay cv: <10%
(Leptin)

inter-assay cv: <15%
intra-assay cv: <10%
(MCP-1)

inter-assay cv: <15%
intra-assay cv: <10%
(NGF)

inter-assay cv: <20%
intra-assay cv: <10%
(TNFα)

技術

multiplexing: suitable

檢測方法

fluorometric (Luminex xMAP)

運輸包裝

wet ice

一般說明

Previously the role of white adipose tissue was thought to be limited to energy storage and internal organ protection. The discovery of Leptin secretion from Adipocytes in 1994 led to the recognition that white adipose tissue is involved in a variety of metabolic and physiological processes. Adipocytes secrete several hormones called Adipokines with functions that include appetite and energy balance, insulin sensitivity and lipid metabolism.

The MILLIPLEX® Human Adipokine Panel 2 is a 9-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum or plasma samples: HGF, IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF and TNF-α. This kit uses a 96-well format, contains a lyophilized standard cocktail, 2 quality controls and can measure up to 38 samples in duplicate.

Panel Type: Metabolism

特異性

Cross Reactivty
Each antibody pair in a panel is highly specific and displays insignificant cross-reactivity to other analytes in the panel.

應用

  • Analytes: IL-1β, IL-6, IL-8, Insulin, Leptin, MCP-1, NGF, TNF-α
  • Recommended Sample Type: Human serum and plasma
  • Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma
  • Assay Run Time: Overnight (16-18 hours) at 2-8°C
  • Research Category: Metabolism
  • Research Subcategory: Endocrine

特點和優勢

Design your multiplex kit by choosing available analytes within this panel.

包裝

Everything you need in a single kit.
96-well plate

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

其他說明

Please contact Technical Service for linearity of dilution.
Sensitivity: See kit protocol for individual sensitives.

法律資訊

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

象形圖

Skull and crossbonesEnvironment

訊號詞

Danger

危險分類

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


分析證明 (COA)

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存取文件庫

Waist-to-height ratio, waist circumference and BMI as indicators of percentage fat mass and cardiometabolic risk factors in children aged 3-7 years.
Sijtsma, A; Bocca, G; L'abee, C; Liem, ET; Sauer, PJ; Corpeleijn, E
Clinical Nutrition (Edinburgh, Scotland) null
Insulin resistance is associated with Fibroblast Growth Factor-23 in stage 3-5 chronic kidney disease patients.
Garland, JS; Holden, RM; Ross, R; Adams, MA; Nolan, RL; Hopman, WM; Morton, AR
Journal of Diabetes and Its Complications null
Altered promoter methylation of PDK4, IL1 B, IL6, and TNF after Roux-en Y gastric bypass.
Kirchner, H; Nylen, C; Laber, S; Barres, R; Yan, J; Krook, A; Zierath, JR; Naslund, E
Surgery for Obesity and Related Diseases : Official Journal of the American Society for Bariatric Surgery null
Yuek Ling Chai et al.
Scientific reports, 11(1), 4010-4010 (2021-02-19)
Cerebrovascular disease (CeVD) and neurodegenerative dementia such as Alzheimer's disease (AD) are frequently associated comorbidities in the elderly, sharing common risk factors and pathophysiological mechanisms including neuroinflammation. Osteopontin (OPN) is an inflammatory marker found upregulated in vascular diseases as well
Maria A Ramos-Roman et al.
Diabetes, 69(8), 1624-1635 (2020-05-10)
Milk production may involve a transient development of insulin resistance in nonmammary tissues to support redistribution of maternal macronutrients to match the requirements of the lactating mammary gland. In the current study, adipose and liver metabolic responses were measured in

相關內容

Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.

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