EQCYTMAG-93K

MILLIPLEX^® Equine Cytokine/Chemokine Magnetic Bead Panel - Immunology Multiplex Assay

The Equine Inflammation / Immunology Bead-Based Multiplex cytokine Assay kit, using the Luminex xMAP technology, enables the simultaneous analysis of 23 cytokine and chemokine biomarkers in equine serum, plasma and cell culture samples.

• 分類程式碼代碼: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

屬性

• 品質等級: 200
• 物種活性: horse
• 製造商／商標名: Milliplex^®
• assay range: accuracy: 74%; (IL-17a); accuracy: 76%; (IL-6); accuracy: 78%; (FGF-2); accuracy: 82%; (G-CSF); accuracy: 83%; (IFNγ); accuracy: 83%; (IL-12 (p70)); accuracy: 85%; (IL-13); accuracy: 86%; (IL-10); accuracy: 88%; (RANTES/CCL5); accuracy: 89%; (IL-8/CXCL8); accuracy: 90%; (IL-2); accuracy: 90%; (MCP-1); accuracy: 91%; (Fractalkine/CS3CL1); accuracy: 92%; (IL-5); accuracy: 93%; (Eotaxin/CCL11); accuracy: 97%; (GRO); sensitivity: 1.4 pg/mL; (IL-6); sensitivity: 10.6 pg/mL; (IL-5); sensitivity: 13.5 pg/mL; (IL-2); sensitivity: 15.4 pg/mL; (IL-17A); sensitivity: 16.3 pg/mL; (FGF-2); sensitivity: 16.7 pg/mL; (IP-10); sensitivity: 165 pg/mL; (IFNγ); sensitivity: 25.3 pg/mL; (IL-18); sensitivity: 29.3 pg/mL; (IL-1β); sensitivity: 3.9 pg/mL; (RANTES/CCL5); sensitivity: 3.94 pg/mL; (GM-CSF); sensitivity: 340 pg/mL; (G-CSF); sensitivity: 355 pg/mL; (IL-4); sensitivity: 39-130 pg/mL; (IL-13); sensitivity: 39.1 pg/mL; (IL-1α); sensitivity: 399 ng/mL; (MCP-1); sensitivity: 457 pg/mL; (Fractalkine/CS3CL1); sensitivity: 49.2 pg/mL; (IL-12 (p70)); sensitivity: 5.2 pg/mL; (TNFα); sensitivity: 62.5 pg/mL; (IL-10); sensitivity: 7.8 pg/mL; (GRO); sensitivity: 74.3 pg/mL; (IL-8/CXCL8); sensitivity: 8.3 pg/mL; (Eotaxin/CCL11); standard curve range: 15-15,000 pg/mL; (IL-17A); standard curve range: 15-15,000 pg/mL; (IL-2); standard curve range: 15-15,000 pg/mL; (IL-5); standard curve range: 15-15,000 pg/mL; (IL-6); standard curve range: 15-15,000 pg/mL; (IP-10); standard curve range: 16.3 pg/mL; (FGF-2); standard curve range: 29-30,000 pg/mL; (IL-1β); standard curve range: 293-300,000 pg/mL; (IL-4); standard curve range: 34-35,000 pg/mL; (IL-18); standard curve range: 340 pg/mL; (G-CSF); standard curve range: 342-350,000 ng/mL; (MCP-1); standard curve range: 39-40,000 pg/mL; (IL-12 (p70)); standard curve range: 39-40,000 pg/mL; (IL-13); standard curve range: 4-4,000 pg/mL; (TNFα); standard curve range: 457 pg/mL; (Fractalkine/CS3CL1); standard curve range: 488-500,000 pg/mL; (IFNγ); standard curve range: 49-50,000 pg/mL; (IL-10); standard curve range: 5-5,000 pg/mL; (GM-CSF); standard curve range: 5-5,000 pg/mL; (RANTES/CCL5); standard curve range: 59-60,000 pg/mL; (IL-1α); standard curve range: 59-60,000 pg/mL; (IL-8/CXCL8); standard curve range: 6-6,000 pg/mL; (GRO); standard curve range: 8.3 pg/mL; (Eotaxin/CCL11)
• 技術: multiplexing: suitable
• 檢測方法: fluorometric (Luminex xMAP)
• 運輸包裝: ambient

描述

一般說明

Inflammation and immune cell response are key to maintaining homeostasis. The more we understand the mechanisms of disease, the more we discover the role that inflammation plays in the etiology of disease. This is as true for horses as it is for humans. Both experience immunological diseases, such as infectious diseases, osteoarthritis, and respiratory diseases, as well as neurologic, metabolic, and cardiovascular diseases and cancer.

MILLIPLEX^® Equine Cytokine/Chemokine Premix Panel is a 23-plex kit used for the simultaneous quantification of any or all the following analytes in equine serum, plasma, or culture supernatant samples: Eotaxin/CCL11, FGF-2, Fractalkine/CS3CL1, G-CSF, GM-CSF, GRO, IFN, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8/CXCL8, IL-10, IL-12 (p70), IL-13, IL-17a, IL-18, IP-10, MCP-1, RANTES/CCL5 and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

特異性

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

應用

• Analytes: Eotaxin/CCL11, FGF-2, Fractalkine/CX3CL1, G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, IL-12 (p70), IL-13, IL-17A, IL-18, IP-10 (CXCL10), MCP-1, RANTES (CCL5), TNF-α
• Recommended Sample Type: Equine serum, plasma, tissue culture supernatant or tissue/cell extracts
• Recommended Sample Dilution: 25 μL per well of undiluted serum or plasma; tissue/cell culture samples may require dilution with an appropriate medium
• Assay Run Time: A primary incubation of 2 hours at room temperature (RT) (20-25°C), or for greater sensitivity, overnight (16-18 hours) at 2-8°C; followed by a 1 hour secondary incubation at RT
• Research Category: Inflammation & Immunology

特點和優勢

Design your multiplex kit by choosing available analytes within this panel.

包裝

96 well plate

儲存和穩定性

Recommended storage for kit components is 2 - 8°C.

法律資訊

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 象形圖: GHS08,GHS05,GHS07,GHS09
• 訊號詞: Danger
• 危險聲明: H302 + H312 + H332,H317,H318,H373,H411
• 防範說明: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• 危險分類: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• 標靶器官: Respiratory Tract
• 儲存類別代碼: 10 - Combustible liquids