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重要文件

CC077

Sigma-Aldrich

Human Collagen Type V

from human placenta, liquid, 1 mg/mL, suitable for cell culture, used for gel formation

同義詞:

Collagen Type V

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About This Item

分類程式碼代碼:
12352202
eCl@ss:
32160405
NACRES:
NA.75

product name

人胶原V型,

生物源

human

品質等級

化驗

95% (SDS-PAGE)

形狀

liquid

製造商/商標名

Chemicon®

濃度

1 mg/mL

技術

cell culture | mammalian: suitable

雜質

<0.5% non-collagen proteins
<1% collagen type III
<2% collagen type I
<2% collagen type IV

輸入

sample type mesenchymal stem cell(s)
sample type induced pluripotent stem cell(s)
sample type pancreatic stem cell(s)
sample type epithelial cells
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type: human embryonic stem cell(s)

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

基因資訊

一般說明

V型胶原是纤维型胶原蛋白,存在于肺、骨、胎膜中,与I型胶原共分布。位于细胞外基质,含量偏低。

應用

人类V型胶原蛋白已用于荧光偏振(FP)分析和表面等离子共振(SPR)技术研究高内皮细胞微静脉蛋白(high endothelial venule protein, hevin)和人V型胶原的相互作用。还用于孵育96孔板,用于测定基质和成纤维细胞裂解物中的蛋白水平。

生化/生理作用

V型胶原可调节角膜和皮肤真皮中的胶原纤维形成。与基质胶原和结构蛋白相互作用,共同维持组织的结构完整性。V型胶原水平偏低会导致角膜透明度的丧失和Ehlers-Danlos综合征(皮肤弹性过度综合征)。

外觀

液体,在0.1 M醋酸(pH 3.0)中。 没有添加防腐剂。

準備報告

通过人胎膜胃蛋白酶提取物的连续盐沉淀和DEAE-纤维素色谱纯化。

儲存和穩定性

将未稀释的分装样品保存于-20°C,最长12个月。请勿解冻和重新冷冻。

其他說明

分子组成:α1(V)]2 α2(V),天然三螺旋。通过SDS-PAGE,ORD测量以及与抗胶原类型特异性单克隆抗体的反应来监测天然螺旋结构的纯度和保留。产品来源:人胎盘,HBsAg和HIV抗体阴性

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

仅供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。

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您可以在文件庫中找到最近購買的產品相關文件。

存取文件庫

The effects of tissue pretreatment and pepsin levels on the isolation of collagens from human placenta.
Klasson, S C, et al.
Collagen and Related Research, 6, 397-408 (1986)
Type V collagen in health, disease, and fibrosis
The Anatomical Record, 299(5), 613-629 (2016)
K Gelse et al.
Advanced drug delivery reviews, 55(12), 1531-1546 (2003-11-19)
The extracellular matrix represents a complex alloy of variable members of diverse protein families defining structural integrity and various physiological functions. The most abundant family is the collagens with more than 20 different collagen types identified so far. Collagens are
Characterization of a novel collagen chain in human placenta and its relation to AB collagen.
Sage, H and Bornstein, P
Biochemistry, 18, 3815-3822 (1979)
R W Glanville et al.
European journal of biochemistry, 95(2), 383-389 (1979-04-02)
Native type IV collagen was isolated from human placenta using pepsin solubilisation followed by fractional salt precipitation and chromatogarphic purification. The native preparation was characterised using amino acid analyses, disc gel electrophoresis, segment-long-spacing crystallites and immunological methods. Two component alpha

文章

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

相關內容

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

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