CBL218-I

Anti-Keratin K3/K76 Antibody, clone AE5

clone AE5, from mouse

• 同義詞: 65 kDa cytokeratin, Cytokeratin-3, CK-3, Keratin-3, K3, Type-II keratin Kb3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: mouse
• 品質等級: 100
• 抗體表格: purified immunoglobulin
• 抗體產品種類: primary antibodies
• 無性繁殖: AE5, monoclonal
• 物種活性: rabbit, bovine
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• 同型: IgG1κ
• NCBI登錄號: XP_002711051
• UniProt登錄號: Q29426
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... KRT3(3850)

描述

一般說明

Cytokeratin 3, also known as 65kDa Cytokeratin or Keratin K3 is a specific keratin found primarily in the corneal epithelium and minorly in other epithelium like that of the esophagus. The corneal epithelium tissue forms the outermost layer of the cornea, which is the clear front covering of the eye. Keratins are divided into two primary types based upon their isoelectric mobility and size, acidic, or type 1:40-55kDa, and basic to neutral or type 2, 56-70kDa. Cytokeratin 3 is a type II cytokeratin. Type II cytokeratins form pairs with other cytokeratins. Cytokeratin 3 is expressed in the corneal epithelium with Cytokeratin 12. Together Cytokeratin 3 and Cytokeratin 12 form molecules known as intermediate filaments. These filaments assemble into strong networks that provide strength and resilience to the corneal epithelium. Mutations in Cytokeratin 3 have been associated with Meesmann′s Corneal Dystrophy a disease characterized by fragility of the anterior corneal epithelium.

免疫原

Whole cells corresponding to Rabbit Keratin, type II cytosleletal 3.

應用

Immunohistochemistry Analysis: A 1:1000 dilution from a representative lot detected Keratin K3/K76 in bovine cornea epithelial cells.
Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in Keratin 3 expressed epithelial cells on the surface of submerged and AL explants, indicating that they were derived from limbal progenitor cells via migration. (Kawakita, T., et al. (2005) Am J Pathol, 167:381-93.).
Western Blotting Analysis: A representative lot was used by an independent laboratory in Rabbit epithelial Keratin lysate. (Schermer, A, et al. (1986) J. Cell Biol., 103: 49-62).
Western Blotting Analysis: A representative lot was used by an independent laboratory in rabbit corneal tissue lysate. (Cooper, D., et al. (1986) J. Biol. Chem., 261: 4646-54).
Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in human limbal stem cells cultured in medium supplemented with human EGF. (Sharifi, A.M., et al. (2010) Biocell. 34(1):53-55).
Immunofluorescence Analysis: A representative lot was used by an independent laboratory in rabbit corneal and limbal tissues, limbal explant, and epithelial outgrowth on human AM. (Wang D.Y., et al. (2003) Invest Ophthalmol Vis Sci. 44(11):4698-704).
Immunofluorescence Analysis: A representative lot was used by an independent laboratory inRabbit corneal epithelial Keratin cells. (Schermer, A, et al. (1986) J. Cell Biol., 103: 49-62).

Research Category
Cell Structure

Research Sub Category
Cytokeratins

This Anti-Keratin K3/K76 Antibody, clone AE5 is validated for use in Western Blotting and Immunohistochemistry and Immunofluorescence for the detection of Keratin K3/K76.

品質

Evaluated by Western Blotting in Rabbit eye (cornea) cell lysate.

Western Blotting Analysis: 0.05 µg/mL of this antibody detected Keratin K3/K76 in 10 µg of Rabbit eye (cornea) cell lysate.

標靶描述

~60 kDa observed

聯結

Replaces: CBL218

外觀

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable