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重要文件

CBL197F

Sigma-Aldrich

Anti-Cytokeratin 14 Antibody, clone LL002, FITC conjugated

clone LL002, Chemicon®, from mouse

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

mouse

品質等級

共軛

FITC conjugate

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

LL002, monoclonal

物種活性

human

製造商/商標名

Chemicon®

技術

immunohistochemistry: suitable (paraffin)

同型

IgG3

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... KRT14(3861)

特異性

Cytokeratin 14, a type I intermediate filament, is one of the two cytokeratins that distinguish stratifying epithelial cell types from simple epithelial cell types. Thus, cytokeratin 14 is expressed by stratifying/keratinocyte cell types but not by simple epithelial cells.

FUSION PARTNER: NS1 myeloma cell line

免疫原

Thyroglobulin conjugated synthetic peptide of extreme carboxy terminus of human keratin 14 (last 15 residues)

應用

Research Category
Cell Structure
Research Sub Category
Cytokeratins
Suitable for staining formalin fixed wax sections or frozen tissues. In order to obtain 100% consistency in staining it is considered essential to either microwave treat fixed tissue sections or alternatively use the more recently recommended high temperature antigen unmasking technique. Prior exposure of fixed tissues to enzyme (trypsin) digestion is deleterious to epitope expression. The fixative recommended for use with CBL 197 is neutral buffered formalin.

Optimal working dilutions must be determined by the end user.
This Anti-Cytokeratin 14 Antibody, clone LL002, FITC conjugated is validated for use in IH(P) for the detection of Cytokeratin 14.

外觀

The conjugate is supplied as a 100 test vial in phosphate buffered saline containing 10mM sodium azide and 1 mg/mL bovine serum albumin. For flow cytometry we recommend using 10μL of conjugate per test.

儲存和穩定性

Store at +4°C protected from light. DO NOT FREEZE. For long term use and storage aliquot conjugate into small volumes and store at +4°C.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Constitution of fibrin-based niche for in vitro differentiation of adipose-derived mesenchymal stem cells to keratinocytes.
Sivan, U; Jayakumar, K; Krishnan, LK
BioResearch Open Access null
Aldo Pezzuto et al.
Journal of thoracic disease, 10(7), E570-E576 (2018-09-04)
In non-small cell lung cancer (NSCLC), there is a consensus regarding the use of liquid biopsy, generally, to detect "druggable" mutations and, in particular, to monitor tyrosine kinase inhibitor (TKI) treatments. However, whether circulating tumor cells (CTCs) are better tools
Antibody markers of basal cells in complex epithelia.
Purkis, P E, et al.
Journal of Cell Science, 97 ( Pt 1), 39-50 (1990)
Propagation and phenotypic preservation of rabbit limbal epithelial cells on amniotic membrane.
Wang, DY; Hsueh, YJ; Yang, VC; Chen, JK
Investigative Ophthalmology & Visual Science null

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