ABT58

Anti-phospho PKA-RII (Ser96) Antibody

from rabbit, purified by affinity chromatography

• 同義詞: PRKAR2A, protein kinase, cAMP-dependent, regulatory, type II, alpha
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, rat
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: western blot: suitable
• NCBI登錄號: NP_032950
• UniProt登錄號: P12367
• 運輸包裝: wet ice
• 目標翻譯後修改: phosphorylation (pSer96)
• 基因資訊: human ... PRKAR2A(5576)

描述

一般說明

cAMP-dependent protein kinase type II-alpha regulatory subunit (PKA-RIIα) is an enzymatic protein belonging to the cAMP-dependent kinase regulatory chain family, a group of regulatory proteins involved in the PKA signaling pathway. This pathway has been shown to have regulatory roles in development, growth, and mortality of various organisms. PKA-RIIα is a subunit of the PKA type II regulatory chain, one of two PKA isoforms, and is involved in the regulation of membrane interactions through its binding to anchoring proteins, such as MAP2 kinase. Before its activation with cAMP, the enzyme is comprised of two catalytic chains and two regulatory chains, which split into two catalytic monomers and one regulatory dimmer after interacting with cAMP.

特異性

The antibody recognizes PKA-RIIalpha when phosphorylated at Ser96.

免疫原

Epitope: Phosphorylated Ser96 of PKA-RIIalpha

KLH-conjugated linear peptide corresponding to mouse PKA-RIIalpha phosphorylated at Ser96.

應用

Research Category
Cell Structure

Signaling

Research Sub Category
Cytoskeletal Signaling

Kinases & Phosphatases

This Anti-phospho PKA-RII (Ser96) Antibody is validated for use in WB for the detection of phospho PKA-RII (Ser96).

品質

Evaluated by Western Blot in Forskolin untreated and treated PC12 cell lysates.

Western Blot Analysis: 1 µg/mL of the antibody detected PKA-RIIalpha (Ser96) in 10 µg of Forskolin untreated and treated PC12 cell lysates.

標靶描述

~ 51 kDa MW observed. The specificity of the target was shown by Lambda phosphatase treatment.

聯結

Replaces: 06-704

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl, with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Forskolin untreated and treated PC12 cell lysates

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable