ABT332

Anti-ZEB2 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Zinc finger E-box binding homeobox 2, Smad interacting-protein 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• UniProt登錄號: O60315
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... ZEB2(9839)

描述

一般說明

Zinc finger E-box-binding homeobox 2 (ZEB2), also known as Smad-interacting protein 1 (SIP1, SMADIP1) and Zinc finger homeobox protein 1b (ZFHX1B, ZFX1B), is a transcriptional inhibitor that binds to DNA sequence 5′-CACCT-3′ in different promoters and interacts with activated SMADs. ZEB2 has been shown to repress the transcription of the E-cadherin gene and induce epithelial-mesenchymal transition, a genetic program controlling cell migration during embryonic development and wound healing in vitro. It is suggested that ZEB2 contributes to tumor progression since it’s been demonstrated to protect cells from DNA damage-induced apoptosis. Recent studies have shown that ZEB2 is often observed in the cytoplasm in numerous cancer tissues, implying that its localization may be regulated in normal and tumor tissues. Mutations in this gene are also associated with Mowat-Wilson syndrome (MWIS), also known as Hirschsprung disease-mental syndrome.

免疫原

KLH-conjugated linear peptide corresponding to human ZEB2.

應用

Detect ZEB2 using this rabbit polyclonal antibody, Anti-ZEB2 Antibody validated for use in western blotting, ICC & Immunofluorescence.

Immunocytochemistry Analysis: 20 µg/mL from a representative lot detected ZEB2 in Jurkat cells.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected ZEB2 in Jurkat cells.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected ZEB2 in 15 µg of Jurkat cell lysate.

標靶描述

~200 kDa observed. Uncharacterized bands may appear in some lysate(s). Uniprot describes 2 isoforms at ~134 kDa and ~136 kDa The calculated molecular weight of this protein is 136 kDa, but can be observed at ~200 kDa due to post-translational modification.

外觀

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Jurkat cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable