ABT21

Anti-Amphiphysin II Antibody

from rabbit, purified by affinity chromatography

• 同義詞: bridging integrator 1, amphiphysin-like, box dependant MYC interacting protein 1, Amphiphysin II, Box-dependent myc-interacting protein 1, Amphiphysin-like protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, mouse
• 物種活性（以同源性預測）: human (based on 100% sequence homology)
• 技術: western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_004296
• UniProt登錄號: O00499
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... AMPH(273)

描述

一般說明

Amphiphysin is a nerve terminal-enriched protein thought to be involved in synaptic vesicle endocytosis. One of three amphiphysin homologs, amphiphysin II may interact with amphiphysin I in order to regulate synaptic vesicle endocytosis. During clathrin-mediated endocytosis, amphiphysin has been reported to bind to a component of the clathrin coat, clathrin assembly protein 2. Mutations in the expression of amphiphysin II may be causal to defects in the endocytosis pathway. Expression of amphiphysin II has been observed in neurons along with some endocrine cell types and spermatocytes.

特異性

The antibody is raised against human amphiphysin II, but recognizes both amphiphysin I and Amphiphysin II (see Molecular Weight Notes).

免疫原

Epitope: Unknown

KLH-conjugated recombinant protein corresponding to human Amphiphysin II.

應用

Detect Amphiphysin II using this Anti-Amphiphysin II Antibody validated for use in WB.

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

Western Blot Analysis: 1:1,000 dilution from a previus lot detected Amphiphysin II in 10 µg of rat brain and mouse brain membrane tissue lysates.

品質

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1:1,000 dilution of this antibody detected Amphiphysin II in 10 µg of mouse brain tissue lysate.

標靶描述

It is well established (Ramjaun, 1997) that both Amphiphysin I and II run substantially slower on SDS-PAGE with a larger apparent molecular weight than the predicted molecular weights.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Mouse brain tissue lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable