ABT1493-AF488

Anti-Cofilin-1 Antibody, Alexa Fluor^™ 488 Conjugate

from rabbit, ALEXA FLUOR^™ 488

• 同義詞: Cofilin-1, 18 kDa phosphoprotein, Cofilin, non-muscle isoform, p18
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702

屬性

• 生物源: rabbit
• 品質等級: 100
• 共軛: ALEXA FLUOR^™ 488
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human, mouse
• 物種活性（以同源性預測）: porcine (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable
• NCBI登錄號: NP_005498
• UniProt登錄號: P23528
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... CFL1(1072)

描述

一般說明

Cofilin-1 (UniProt P23528; also known as 18 kDa phosphoprotein, Cofilin non-muscle isoform, p18) is encoded by the CFL1 (also known as CFL) gene (Gene ID 1072) in human. Cofilin-1 (non-muscle cofilin), cofilin-2 (muscle cofilin), and actin-depolymerizing factor (ADF) play an essential role in regulating actin filament dynamics and reorganization by stimulating the severance and depolymerization of actin filaments. The three cofilin family members share 70% to 81% sequence identity, exhibit similar biological functions, and display distinct but overlapping expression patterns. Cofilins and ADF bind actin monomers and filaments (G-actin and F-actin) and increase the number of actin monomers and filament fragments, facilitating filament turnover and treadmilling at key locations in migrating cells. Cofilins and ADF are inactivated by phosphorylation at Ser3 by LIM-kinases (LIMKs) and testicular protein kinases (TESKs) and are reactivated by dephosphorylation by the slingshot (SSH) family of protein phosphatases and chronophin. Cfl1-knockout results in mouse embryonic lethality at E11.5 12.5 due to aberrant neural tube closure and defective neural crest cell migration, while Adf-deficient mice are viable with normal brain appearance, but suffer from corneal defects that cause blindness in adult mice.

特異性

This Alexa Fluor^™ 488-conjugated polyclonal antibody targets cofilin-1 N-terminal end sequence conserved among bovine, human, mouse, porcine, and rat species.

免疫原

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-termnal end sequence of human cofilin-1.

應用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected cofilin-1 in NIH/3T3 cells.

The unconjugated antibody (Cat. No. ABT1493) is also available for immunocytochemistry, immunoprecipitation, and Western blotting applications.

Research Category
Cell Structure

This rabbit polyclonal Anti-Cofilin-1 Antibody, Alexa Fluor^™ 488 Conjugate, Cat. No. ABT1493-AF488 is validated for use in Immunocytochemistry for the detection of cofilin-1.

品質

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected cofilin-1 in HeLa cells.

標靶描述

~19 kDa observed. 18.39/18.37/184.3/18.39/18.40 kDa (bovine/human/mouse/porcine/rat) calculated (Met1 removed).

外觀

Affinity purified.

Purified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

ALEXA FLUOR is a trademark of Life Technologies

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable