ABT1358M

Anti-Versican Antibody, Alpha Domain

serum, from rabbit

• 同義詞: Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat
• 包裝: antibody small pack of 25 μL
• 技術: immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; radioimmunoassay: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_001164029
• UniProt登錄號: Q9ERB4
• 目標翻譯後修改: unmodified
• 基因資訊: rat ... Vcan(114122)

描述

一般說明

Versican core protein (UniProt: Q9ERB4; also known as Chondroitin sulfate proteoglycan core protein 2, Glial hyaluronate-binding protein, GHAP, Large fibroblast proteoglycan, PG-M) is encoded by the Vcan (also known as Cspg2) gene (Gene ID: 114122) in rat. Versican is a large multi-domain chondroitin sulphate proteoglycan that is secreted by fibroblasts and other types of cells. Versican is present in most of the retina including the outer nuclear layer, the outer plexiform layer, and the inner plexiform layer. It is synthesized with a 20 amino acid signal peptide, which is subsequently cleaved. Veriscan plays a role in intercellular signaling and in connecting cells with the extracellular matrix and is also involved in axon regeneration, central nervous system and skeletal muscle development, and cell adhesion. It has unique N- and C-terminal globular regions, each with multiple motifs. It contains one C-type lectin domain, two EGF-like domains, one Ig-like V-type domain, two link domains and one Sushi (CCP/SCR) domains. It also has a N-terminal hyaluronic acid (HA) binding domain that has homology with the G1 and G2 domains of aggrecan. Four isoforms of versican termed as V0, V1, V2, and V3, have been reported with molecular weights of 370, 263, 180, and 74 kDa, respectively. Each isoform contains different lengths of the GAG binding region. V0 contains two GAG binding regions called CSalpha and CSbeta domains. V1 isoform has only CSbeta and V2 isoform has only CSalpha domain. V3 isoform contains only G1 and G3 domains and lacks GAG attachment sites. Defects in Vcan gene result in Wagner syndrome type 1, a type of vitreoretinopathy characterized by an optically empty vitreous cavity with fibrillary condensations and a pre-retinal avascular membrane.

特異性

This rabbit polyclonal antibody detects versican in rat eye sections.

免疫原

Epitope: unknown

GST-tagged recombinant fragment corresponding to the first 321 amino acids of rat Versican.

應用

Anti-Versican, Alpha Domain, Cat. No. ABT1358, is a rabbit polyclonal antibody that detects versican core protein that has been tested for use in Immunohistochemistry (Paraffin), Radioimmunoassay, and Western Blotting.

Radioimmunoassay Analysis: A representative lot detected Versican, Alpha Domain in Postnatal rat brain (Milev, P., et. al. (1998). Biochem Biopys Res Commun. 247(2):207-12).

Immunohistochemistry Analysis: A representative lot detected Versican, Alpha Domain in Embryonic day 16 rat eye, P0, P9 and adult rat retina (Popp, S., et. al. (2004). Exp Eye Res. 79(3):351-6) and Embryonic day 16, 19 rat brain and postnatal day 7 cerebellum, embryonic day 19 spinal cord (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

Western Blotting Analysis: A representative lot detected Versican, Alpha Domainin in purified proteoglycans from a PBS extract of 7-day rat brain with chondroitinase ABC treatment (Popp, S., et. al. (2003). Dev Dyn. 227(1):143-9).

Research Category
Cell Structure

品質

Evaluated by Immunohistochemistry in rat eye retina tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected Versican, Alpha Domain in rat eye retina tissues.

標靶描述

300.01 kDa calculated.

外觀

Unpurified

Rabbit polyclonal antiserum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable