ABS527

Anti-Sphk2, C-term Antibody

serum, from rabbit

• 同義詞: Sphingosine kinase 2, SK 2, SPK 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse
• 技術: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_001166032
• UniProt登錄號: Q9JIA7
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Sphk2(56632)

描述

一般說明

Sphingosine kinase 2 (Sphk2), along with its sister enzyme Sphk1, is an important kinase needed to form the important intra and extracellular signaling lipid mediator and neuroprotective factor SPP/S1P (sphingosine 1-phosphate). Sphk2 is important in blood vessel development, brain development, as well as an anti-apoptotic factor, and it mediates many proliferation mediated responses such as those surrounding insulin and its mitogenic ability. Sphk2 is localized to the lysosome membrane primarily but can also be found in the cytoplasm. Interestingly, recent research indicates that ApoE regulates the secretion Sphingosine 1-phosphate in the brain, and SPP/S1P in turn positively regulates glutamate secretion and synaptic strength in hippocampal neurons, thus Sphk1/2 plays an important role in synaptic plasticity and neuronal activity connectivity in the brain as well.

免疫原

Epitope: C-terminus

Recombinant protein corresponding to the C-terminus of mouse Sphk2, C-term.

應用

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-Sphk2, C-term Antibody is validated for use in Western Blotting and Immunoprecipitation and Immunocytochemistry for the detection of Sphk2, C-term.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Sphk2 in 10 µg of mouse lung tissue lysate.
Western Blotting Analysis: A representative lot detected Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Sphk2 in mouse BMMC tissue lysate (Olivera, A., et al. (2006). JBC. 281(5):2515-2525).
Immunocytochemistry Analysis: A representative lot detected Sphk2 in Huvec cells (Venkataraman, K., et al. (2006) Biochem. J. 397:461-471).

品質

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Sphk2, C-term in 10 µg of RAW264.7 cell lysate.

標靶描述

~65 kDa observed, uncharacterized bands may be observed at higher molecular weights

外觀

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1