ABS2192

Anti-mPTGES-1

from rabbit

• 同義詞: Prostaglandin E synthase, EC:5.3.99.3, Microsomal glutathione S-transferase 1-like 1, MGST1-L1, Microsomal prostaglandin E synthase 1, MPGES-1, p53-induced gene 12 protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: polyclonal
• application: IF; IHC; WB
• 物種活性: mouse, human
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: unpurified
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse, human
• 物種活性（以同源性預測）: rat (based on high sequence homology)
• 技術: immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_004869
• UniProt登錄號: O14684
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PTGES(9536)

描述

一般說明

Prostaglandin E synthase (UniProt: O14684; also known as EC:5.3.99.3, Microsomal glutathione S-transferase 1-like 1, MGST1-L1, Microsomal prostaglandin E synthase 1, MPGES-1, p53-induced gene 12 protein) is encoded by the PTGES gene (Gene ID: 9536) in human. mPGES -1 is a multi-pass membrane protein that is integral membrane, inducible and glutathione-dependent terminal synthase in the prostanoid biosynthesis pathway and acts downstream cyclooxygenases (COX) catalyzing the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2). Functionally, mPGES-1 is coupled to COX-2 and the induction of these two enzymes by pro-inflammatory cytokines, growth factors or endotoxin leads to a surge of PGE2 synthesis by inflammatory cells. It is strongly expressed with COX-2 in synovial fibroblasts and macrophages. In cases of osteoarthritis, mPGES-1 and COX-2 are strongly expressed in the chondrocytes and the synovial lining cells. mPGES-1 also play a role in the pathogenesis of ischemic stroke and many neurodegenerative diseases. In Alzheimer disease brains higher expression of both COX-2 and mPGES-1 is observed. Higher expression of mPGES has also been reported in several cancers and this higher expression has been correlated with vascular invasion and worse prognosis in colorectal cancer. A549 and DU145 xenografts lacking mPGES-1 display significantly reduced growth rates. (Ref.: Korotkova, M., and Jakobsson, PJ (2014). Basic Clin. Pharmacol. Toxicol. 114, 64-69).

特異性

This polyclonal antibody specifically detects mPGES-1 in human.

免疫原

His-tagged full-length recombinant human microsomal prostaglandin E synthase.

應用

Detect Prostaglandin E synthase using this rabbit polyclonal Anti-mPTGES-1, Cat. No. ABS2192 that has been tested in Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunofluorescence, and Western Blotting.

Immunohistochemistry Analysis: A 1:5,000 dilution from a representative lot detected mPTGES-1 in human tonsil and human placenta tissue.

Western Blotting Analysis: A representative lot detected mPTGES-1 in human chondrocytes (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9) and wild-type mixed glial cultures (Straccia, M., et. al. (2013). Glia. 61(10):1607-19).

Immunofluorescence Analysis: A representative lot detected mPTGES-1 in Wild-type mixed glial cultures (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9).

Western Blotting Analysis: A representative lot detected mPTGES-1 in PNE inhibitedmPGES-1proteinexpressioninIL-1 -stimulated SK-N-SH cells (Olajide, O.A., et. al. (2014). J Ethnopharmacol. 152(2):377-83).

Immunohistochemistry Analysis: A representative lot detected mPTGES-1 in fresh frozen brain tissue (Tuure, L., et. al. (2015). Scand J Rheumatol. 44(1):74-9).

品質

Evaluated by Western Blotting in recombinant microsomal Prostaglandin E synthase 1 protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected mPTGES-1 in recombinant microsomal Prostaglandin E synthase 1 protein.

標靶描述

~17 kDa observed; 17.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Format: Unpurified

Rabbit polyclonal antiserum without azide.

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable