ABS1587

Anti-Ribophorin I/RPN-I Antibody

serum, from rabbit

• 同義詞: Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat, canine, human
• 物種活性（以同源性預測）: nonhuman primates (based on 100% sequence homology), porcine (based on 100% sequence homology), monkey (based on 100% sequence homology)
• 技術: western blot: suitable
• NCBI登錄號: NP_002941
• UniProt登錄號: P04843
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... RPN1(6184)

描述

一般說明

Dolichyl-diphosphooligosaccharide-protein glycosyltransferase subunit 1 (EC 2.4.99.18; UniProt P04843; also known as Dolichyl-diphosphooligosaccharide-protein glycosyltransferase 67 kDa subunit, Ribophorin I, RPN-I, Ribophorin-1) is encoded by the RPN1 gene (Gene ID 6184) in human. Ribophorin I is an ER transmembrane protein and a subunit of the mammalian Oligosaccharyltransferase (OST) complex. Ribophorin I binds newly synthesized target proteins and delivers them to the catalytic core of the OST complex. Association of ribophorin I with newly synthesized membrane proteins was observed after the exit of the nascent chain from the Sec61 translocon, and it was unaffected by the N-glycosylation status. Ribophorin I is reported to preferentially interact with misfolded over native ribonuclease A. In addition, Ribophorin I is shown to complex with another ER protein malectin and enhance malectin′s ability to prevent the secretion of the folding-defective proteins.

免疫原

Epitope: C-terminus.

KLH-conjugated linear peptide corresponding to the C-terminal sequence of human Ribophorin I/RPN-I.

應用

Research Category
Signaling

Research Sub Category
Organelle & Cell Markers

This Anti-Ribophorin I/RPN-I Antibody is validated for use in Western Blotting for the detection of Ribophorin I.

Western Blotting Analysis: An 1:5,000 dilution from a representative lot detected ribophorin I in in 25 µg of HeLa cell lysate (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC).
Western Blotting Analysis: A representative lot detected ribophorin I in the alkali-extracted and Triton X-114-selected integral membrane fraction derived from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I in both the Brij35-sensitive (BrS) and Brij35-resistant (BrR) fraction of HeLa ER membrfane proteins (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I associated with the rRNA-free poly(A) messenger ribonucleoproteins (mRNPs) purified by oligo(dT) from UV-cross-linked canine pancreas rough microsomes (RM) preparation (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
Western Blotting Analysis: A representative lot detected ribophorin I in both the smooth and rough microsomes sub-fractionated from a rat liver microsome preparation (Menon, A.K., et al. (2000). Curr. Biol. 10(5):241-252).
Western Blotting Analysis: A representative lot detected ribophorin I in canine rough microsome (RM) fractions obtained by sucrose gradient centrifugation of digitonin- or DHPC-solubilized canine RM preparation with or without ribosomes stripping by EDTA treatment (Potter, M.D., and Nicchitta, C.V. (2000). J. Biol. Chem. 275(3):2037-2045).

品質

Evaluated by Western Blotting in canine pancreas tissue rough microsome lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Ribophorin I/RPN-I in 2.5 µL of canine pancreas tissue rough microsome lysate.

標靶描述

~68 kDa observed.

外觀

Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable