ABN306

Anti-MANF Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Mesencephalic astrocyte-derived neurotrophic factor, arginine-rich mutatedin early stage tumor, ARMET, ARP
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, mouse, human
• 技術: immunohistochemistry: suitable; western blot: suitable
• UniProt登錄號: P55145
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... MANF(7873)

描述

一般說明

MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson’s disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.

特異性

This antibody does not cross-react with CDNF.

免疫原

KLH-conjugated linear peptide corresponding to human MANF.

應用

Detect the MANF protein using this Anti-MANF validated for use in WB & IHC.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected MANF in human midbrain and rat brain tissues.

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

品質

Evaluated by Western Blot in PC12 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected MANF in 15 µg/mL of PC12 cell lysate.

標靶描述

~18 kDa observed. The calculated molecular weight is 21 kDa, however MANF has been shown as an ~18 kDa band in western blots. (Mizobuchi, N., et al. (2007). Cell Structure and Function. 32:41-50.). Uncharacterized bands may appear at ~25 kDa and ~100 kDa in some lysates.

外觀

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
PC12 cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable