ABN2268M

Anti-proSAAS

serum, from rabbit

• 同義詞: IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: rat, mouse, human
• 技術: immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• 同型: IgG
• NCBI登錄號: NP_038920
• UniProt登錄號: Q9QXV0
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PCSK1N(27344) ; mouse ... Pcsk1N(30052)

描述

一般說明

ProSAAS (UniProt: Q9QXV0; also known as IA-4, Proprotein convertase subtilisin/kexin type 1 inhibitor, Proprotein convertase 1 inhibitor, pro-SAAS) is encoded by the Pcsk1n gene (Gene ID: 30052) in murine species. ProSAAS is a small secretory protein that is primarily expressed in neural and neuroendocrine tissues and acts as an anti-aggregant chaperone. Its presence appears to be restricted only to vertebrates. Mammalian proSAAS does not undergo either glycosylation or phosphorylation, but undergoes proteolytic cleavage at several pairs of basic residues that leaves its core region intact. ProSAAS is known to interact with proprotein convertase subtilisin/kexin type 1 (PCSK1) via the C-terminal inhibitory domain (aa 219-258). In the neural tissues proSAAS exhibits neuroprotective effects and is shown to block alpha-synuclein-induced cytotoxicity in cultures of dopaminergic neurons and in SH-SY5Y cells. In vitro studies have shown that proSAAS can also prevent the fibrillation of amyloid beta 1-42 peptide in a dose-dependent manner and residues 97-180 are sufficient for its anti-aggregation function against amyloid peptides. Inclusion of recombinant proSAAS in the medium of Neuro2a cells is shown to block the neurocytotoxic effect of amyloid peptide 1-42.

特異性

This rabbit polyclonal antibody detects proSAAS in human, mouse, and rat cells. It targets an epitope within the N-terminal half.

免疫原

His-tagged recombinant fragment corresponding to first 180 amino acids from murine ProSAAS.

應用

Anti-proSAAS Antibody, Cat. No. ABN2268, is a highly specific rabbit polyclonal antibody that targets ProSAAS and has been tested in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected proSAAS in AtT-20 cells. (Courtesy of Dr. Iris Lindberg @ University of Maryland).

Immunofluorescence Analysis: A representative lot detected proSAAS in Immunofluorescence applications (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15; Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30; Guest, P.C., et. al. (2002). J Endocrinol. 175(3):637-47).

Western Blotting Analysis: A representative lot detected proSAAS in A immunoprecipitated from mouse brain lysates (Hoshino, A., et. al. (2014). J Neurochem. 128(3):419-30). Notes: Treating blots with 1% glutaraldehyde in PBS for 30 min prior to blocking significantly increased the proSAAS signal and reduces the background.

Immunocytochemistry Analysis: A representative lot detected proSAAS in SH-SY5Y cells (Jarvela, T.S., et. al. (2016). Proc Natl Acad Sci USA. 113(32):E4708-15).

品質

Evaluated by Western Blotting in His-tagged mouse proSAAS recombinant protein.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected His-tagged mouse proSAAS recombinant protein. Note: For most biological samples a range of 1:1000 for Western blotting and 1:500 for IHC is recommended.

標靶描述

~19 kDa observed; 27.27 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable