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Merck
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重要文件

ABN1452

Sigma-Aldrich

Anti-TRIO

from rabbit, purified by affinity chromatography

同義詞:

Triple functional domain protein, EC: 2.7.11.1.

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

human

包裝

antibody small pack of 25 μg

技術

immunohistochemistry: suitable (paraffin)
western blot: suitable

同型

IgG

NCBI登錄號

UniProt登錄號

運輸包裝

ambient

目標翻譯後修改

unmodified

基因資訊

human ... TRIO(7204)

一般說明

Triple functional domain protein (UniProt: O75962; also known as EC: 2.7.11.1, PTPRF-interacting protein, TRIO) is encoded by the TRIO gene (Gene ID: 7204) in human. TRIO is a complex protein that is widely expressed in human tissue, with highest levels reported in heart, skeletal muscle, and brain. Five isoforms of TRIO have been reported that are produced by alternative splicing. It contains three catalytic domains, two tandem Dbl Homology (DH) and pleckstrin homology (PH) domains, and a serine/Threonine kinase domain. The DH-PH module is involved in guanine nucleotide exchange and the activation of the small GTPases of the Rho family. The DH1-PH1 domain (GEF1) is reported to activate the Rho G and Rac1, whereas DH2-PH2 (GEF2) is involved in the activation of RhoA. TRIO is involved in coordinating actin remodeling, which is necessary for cell migration and growth. In developing hippocampal neurons it limits dendrite formation, without affecting the establishment of axon polarity. After dendrites are formed, it is reported to be involved in the control of synaptic function by regulating the endocytosis of AMPA-selective glutamate receptors at CA1 excitatory synapses. Mutations in TRIO gene have been linked to mental retardation and microcephaly. (Ref.: Debant, A. et al. (1996). Proc. Natl. Acad. Sci, USA. 93(11); 5466-71; Bellanger, JM. Et al. (2000). Nat. Cell Biol. 2(12); 888-92).

特異性

This rabbit polyclonal antibody detects human Triple functional domain protein (TRIO). It targets an epitope within 17 amino acids from the C-terminal half. The immunogen sequence is conserved in all 5 isoforms.

免疫原

KLH-conjugated liner peptide corresponding to 17 amino acids from the C-terminal half of human Triple functional domain protein.

應用

Anti-TRIO Antibody, Cat. No. ABN1452, is a highly specific rabbit polyclonal antibody that targets Triple functional domain protein (TRIO) and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected TRIO in human cerebral cortex, human pancreas, and human testis tissue.

品質

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRIO in 10 µg of HEK293 cell lysate.

標靶描述

~300 kDa observed; 346.90 kDa calculalted. Uncharacterized bands may be observed in some lysate(s).

其他說明

Concentration: Please refer to lot specific datasheet.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

does not flash

閃點(°C)

does not flash


分析證明 (COA)

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