ABF1820

Anti-Influenza A nucleoprotein

serum, from rabbit

• 同義詞: Nucleoprotein, Nucleocapsid protein, Protein N
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: virus, influenza A virus
• 包裝: antibody small pack of 25 μL
• 技術: immunofluorescence: suitable; western blot: suitable
• 同型: IgG
• GenBank登錄號: AAD12236 (Translation)
• UniProt登錄號: Q9WLG4
• 目標翻譯後修改: unmodified
• 基因資訊: SARS coronavirus ... N(1489678)

描述

一般說明

Nucleoprotein (UniProt: Q9WLG4; also known as Nucleocapsid protein, Protein N) is encoded by the NP gene in Influenza A virus (strain A/Victoria/3/1975 H3N2). The genomes of all negative-strand RNA viruses are coated by the viral nucleoprotein. Together with the viral RNA-dependent RNA polymerase, these ribonucleoprotein complexes form the minimal functional unit for all viral transcription and replication. Influenza A virus has a negative-sense RNA genome that encodes a single-strand RNA-binding nucleoprotein. The primary function of the nucleoprotein is to encapsidate the virus genome for the purposes of RNA transcription, replication, and packaging. It is considered to be a critical factor in the viral infectious cycle in switching influenza virus RNA synthesis from transcription mode to replication mode. It also functions as a key adapter molecule between virus and host cell processes. It achieves this through its ability to interact with a wide variety of viral and cellular macromolecules, including RNA, itself, two subunits of the viral RNA-dependent RNA polymerase and the viral matrix protein. Nuceloprotein can also interact with cellular polypeptides, including actin, components of the nuclear import and export apparatus and a nuclear RNA helicase. Influenza A nucleoprotein has an unconventional nuclear localization signal (NLS) (aa 1-18) and a bipartite NLS (aa 1980216). (Ref.: Portela, A., and Digard, P. (2002). J. Gen. Virol. 83(4); 723-34; de Lucas, S., et al. (2010). J. Virol. 84(15); 7603-7612).

特異性

This rabbit polyclonal antibody detects influenza A virus nucleoprotein in virus infected cells.

免疫原

Epitope: unknown

Hig-tagged full length recombinant nucleoprotein from Influenza A virus.

應用

Anti-Influenza A nucleoprotein, Cat. No. ABF1820, is a rabbit polyclonal antibody that detects influenza virus nucleoprotein and has been tested for use in Immunofluorescence and Western Blotting.

Immunofluorescence Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB).

Western Blotting Analysis: A 1:3,000 dilution from a representative lot detected Influenza A nucleoprotein in A549 cells were infected with A/PR/8/34 strain (Courtesy of Dr. Amelia Nieto at CNB).

Western Blotting Analysis: A representative lot detected Influenza A nucleoprotein in Western Blotting applications (Jorba, N., et. al. (2009). PLoS Pathog. 5(5):e1000462; Lukarsk, M., et. al. (2017). Nature. 541(7635):117-121; Tripathi, S., et. al. (2015). Cell Host Microbe. 18(6):723-35).

Immunofluorescence Analysis: A representative lot detected Influenza A nucleoprotein in Immunofluorescence applications (Marcos-Villar, L., et. al. (2018). Sci Rep. 8(1):1230; Rodriguez-Fandsen, A., et. al. (2016). Sci Rep. 6:20744).

Research Category
Inflammation & Immunology

品質

Evaluated by Western Blotting in A549 cells infected with A/Victoria/3/75 strain of influenza virus.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected Influenza A nucleoprotein in A549 cells infected with A/Victoria/3/75 strain of influenza virus.

標靶描述

~56 kDa observed; 56.10 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外觀

Unpurified

Rabbit polyclonal antiserum in 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

法律資訊

GenBank is a registered trademark of United States Department of Health and Human Services

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.