ABE477

Anti-BRD2 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Bromodomain-containing protein 2, O27.1.1, Really interesting new gene 3 protein
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human, rat
• 技術: immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001106653.1
• UniProt登錄號: P25440
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... BRD2(6046)

描述

一般說明

Bromodomain-containing protein 2 (BRD2) is a member of the BET subfamily of bromodomain proteins. These proteins contain two tandem N-terminal bromodomains and a single C-terminal extra-terminal domain. Studies have shown BRD2 to be associated with several regulators of transcription (eg. TFIID and Swi/Snf complexes) in addition to its involvement in guiding cell cycle gene expression through its binding to multiple E2F. BRD2 is hypothesized to function in mammalian development by regulating chromatin structure and transcription. Consistent with this hypothesis, studies have shown BRD2 to bind histone H4 via acetylated Lys12. BRD2 mutants have been associated with increased susceptibility to juvenile myoclonic epilepsy.

特異性

This antibody is expected to cross react with isoforms 1 and 2 of BRD2, based upon sequence homology.

免疫原

GST-tagged recombinant protein corresponding to a region near the C-terminus of human BRD2.

應用

Anti-BRD2 Antibody is a highly specific rabbit polyclonal antibody, that targets BRD2 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: A 1:500 dilution of this antibody from a representative lot detected BRD2 in human testis tissues.

Immunohistochemistry Analysis: A 1:2,000 and a 1:500 dilution from a representative lot detected BRD2 in rat testis tissues.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Chromatin Biology

品質

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected BRD2 in 10 µg of Jurkat cell lysate.

標靶描述

~110 kDa observed. Uniprot describes two isoforms at 88 kDa (isoform 1) and 92 kDa (isoform 2). Uncharacterized band(s) may be observed in some cell lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable