ABE278

Anti-MILI Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Piwi-like protein 2
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: rat, mouse
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• NCBI登錄號: NP_067283
• UniProt登錄號: Q8CDG1
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Piwil2(57746) ; rat ... Piwil2(306011)

描述

一般說明

MILI, MIWI, and MIWI2 belong to the Piwi family of proteins. piRNAs and Piwi proteins are implicated in transposon control and are linked to transposon methylation in mammals. MILI, in particular, directly binds with piRNAs in the cytoplasm and is required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. MILI plays an essential role in spermatogenesis, meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells.

免疫原

KLH-conjugated linear peptide corresponding to mouse MILI.

應用

Anti-MILI Antibody is a Rabbit Polyclonal Antibody for detection of MILI also known as Piwi-like protein 2 & has been validated in WB, ICC, IHC & IP.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in male primordial germ cells. (Aravin, A.A., et al. (2008). Mol Cell. 31(6):785-799.)

Immunohistochemistry Fluoresence Analysis: A representative lot was used by an independent laboratory in embyronic testis cross sections. (Vagin, W., et al. (2009). Genes Dev. 23(15):1749-1762.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory. (Aravin, A.A., et al. (2007). Science. 316(5825):744-747.)

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

品質

Evaluated by Western Blot in RAW264.7 cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected MILI in 10 µg of RAW264.7 cell lysate.

標靶描述

~109 kDa observed. An uncharacterized bands at ~30, 55, and 60 kDa may be observed in some lysates.

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
RAW264.7 cell lysate

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable