ABE1958

Anti-Zic1/2

from rabbit

• 同義詞: Zinc finger protein of the cerebellum 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: unpurified
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: human, mouse
• 技術: ChIP: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• NCBI登錄號: NP_033599
• UniProt登錄號: P46684
• 運輸包裝: ambient
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Zic1(22771)

描述

一般說明

Zinc finger protein ZIC 1 (UniProt: P46684; also known as Zinc finger protein of the cerebellum 1) is encoded by the Zic 1 (also known as Zic) gene (Gene ID: 22771) in murine species. Zic 1 is a member of the GLI C2H2-type zinc-finger protein family. Zic 1 is expressed in the central nervous system and in osteoblasts. A high level expression is seen in the cerebellum, while a low level expression is seen in the olfactory bulb, diencephalon, and brainstem. It acts as a transcriptional activator that is involved in neurogenesis and plays important roles in the early stage of organogenesis of the CNS, as well as during dorsal spinal cord development and maturation of the cerebellum. The C2H2-type 3, 4 and 5 zinc finger domains of Zic 1 are considered to be essential for transcription activation and for Zic 1 interaction with MyoD family inhibitor domain-containing (MDFIC) protein. Zic 1 is also shown to be involved in the spatial distribution of mossy fiber (MF) neurons within the pontine gray nucleus (PGN). It plays a role in the regulation of MF axon pathway choice and promotes MF migration towards ipsilaterally-located cerebellar territories.

特異性

This polyclonal antibody detects Zic1 protein in mouse and human.

免疫原

A linear peptide corresponding to 20 amino acids from the C-terminal end of mouse Zic 1 protein.

Epitope: C-terminus

應用

Anti-Zic1/2, Cat. No. ABE1958, is a highly specific rabbit polyclonal antibody that targets Zinc finger protein ZIC 1 and has been tested in Chromatin Immunoprecipitation (ChIP), Immunocytochemistry, Immunofluorescence, and Western Blotting.

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Zic1/2 in mouse cerebellum (Frank, C.L., et. al. (2015). Nat Neurosci. 18(5):647-56).

Immunofluorescence Analysis: A representative lot detected Zic1/2 in postmitotic granule cells (Kim, J.Y., et. al. (2003). Dev Biol. 263(1):50-66) and adult mouse cerebellum (Koirala, S., et. al. (2009). J Neurosci. 29(23):7439-49).

Immunocytochemistry Analysis: A representative lot detected Zic1/2 in Immunocytochemistry applications (Kenney, A.M., et. al. (2000). Mol Cell Biol. 20(23):9055-67).

Research Category
Epigenetics & Nuclear Function

品質

Evaluated by Western Blotting in human cerebellum tissue lysate.

Western Blotting Analysis: 1:500 dilution of this antibody detected Zic1/2 in 10 µg of human cerebellum tissue lysate.

標靶描述

~53 kDa observed; 48.33 kDa calculated for Zic1; 54.95 kDa calculated for Zic2. Uncharacterized bands may be observed in some lysate(s).

外觀

Unpurified

Format: Unpurified

Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1