ABE1363

Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Histone H2A.Z, H2A/z
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• 無性繁殖: polyclonal
• application: ChIP; DB; ELISA; ICC; WB
• 物種活性: mouse, human
• 技術: ChIP: suitable (ChIP-seq); ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• citations: 7

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: purified antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: mouse, human
• 技術: ChIP: suitable (ChIP-seq); ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_002097
• UniProt登錄號: P0C0S5
• 運輸包裝: wet ice
• 目標翻譯後修改: acetylation (Lys5,Lys7,Lys11)
• 基因資訊: human ... H2AFZ(3015)

描述

一般說明

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

特異性

Human, mouse, wide range expected.

免疫原

KLH-conjugated linear peptide corresponding to human acetyl Histone H2A.Z (Lys 5, 7, 11) near the N-terminus.

應用

Chromatin Immunoprecipitation Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from HeLa cells.
Dot Blot Analysis: 0.05 ug/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in H2A.Z peptides.
Chromatin Immunoprecipitation (Seq.) Analysis: A representative lot immunoprecipitated acetyl Histone H2A.Z (Lys 5, 7, 11) using chromatin from K562 cells.
Immunocytochemistry Analysis: A representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in HeLa cells.

This Anti-acetyl Histone H2A.Z (Lys 5, 7, 11) Antibody is validated for use in Western Blotting and Immunocytochemistry and Chromatin Immunoprecipitation (ChIP) and ChIP-seq and Dot Blot and ELISA for the detection of acetyl Histone H2A.Z (Lys 5, 7, 11).

品質

Western Blotting Analysis: 0.5 µg/mL from a representative lot detected acetyl Histone H2A.Z (Lys 5, 7, 11) in 10 µg of sodium butyrate treated HeLa acid extract.

標靶描述

~16 kDa observed

外觀

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide and 0.05% ProClin300.

其他說明

Concentration: Please refer to lot specific datasheet.

安全資訊

• 象形圖: GHS07
• 訊號詞: Warning
• 危險聲明: H317
• 防範說明: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• 危險分類: Skin Sens. 1
• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable