ABE1361

Anti-NuMA1 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Nuclear mitotic apparatus protein 1, NuMA protein, SP-H antigen
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001273490
• UniProt登錄號: Q14980
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... NUMA1(4926)

描述

一般說明

NuMA1 or NuMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NuMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NuMA1 resides in the nuclear matrix during interphase. NuMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NuMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis.

特異性

Recognizes NuMA1

免疫原

Three different peptides corresponding to multiple locations of NuMA1 isoform 1. Peptide, region between amino acids 1-240. Peptide 2, amino acids 1300-1500. Peptide 3, amino acids 1860-2115. Note that these peptides are also found in isoform 2.

應用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot was reported to detect NuMA1 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Chromatin Biology

This Anti-NuMA1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of NuMA1.

品質

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected NuMA1 in a A431 cell lysate.

標靶描述

~ 238 kDa observed

外觀

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS, 50% glycerol, and 0.05% sodium azide.

儲存和穩定性

Store at -20°C

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 2