ABD71

Anti-FOXC1 Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3, FREAC-3
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: mouse (based on 100% sequence homology)
• 技術: immunocytochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_001444
• UniProt登錄號: Q12948
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... FOXC1(2296)

描述

一般說明

Forkhead box protein C1 (FOXC1) is a member of the family of forkhead transcription factors. These proteins are characterized by the forkhead domain--a 110 amino acid domain that binds to DNA molecules. FOXC1 has been implicated in the development of the eye; disruption of the FOXC1 gene has been linked to a number of glaucoma-related diseases such as Axenfeld-Rieger syndrome.

特異性

Other homologies: Rat (71% sequence homology).

免疫原

KLH-conjugated linear peptide corresponding to human FOXC1.

應用

Anti-FOXC1 Antibody is a Rabbit Polyclonal Antibody for detection of FOXC1 also known as Forkhead box protein C1, Forkhead-related protein FKHL7, FREAC-3 & has been validated in WB, ICC.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot Analysis: A representative lot of this antibody detected FOXC1 in HeLa, HEK293, THP1, and MDA-MB-468 cell lysates, and in human bone marrow tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected FOXC1 in HeLa cells. This antibody positively stains the nucleus, with some cytoplasm staining. This staining pattern has also been observed by an independent laboratory (Berry, F. B., et al. (2002). J Biol Chem. 277(12):10292-10297.).

品質

Evaluated by Western Blot in THP1 cell lysate.

Western Blot Analysis: 0.025 µg/mL of this antibody detected FOXC1 in 10 µg of THP1 cell lysate. No Tween^® reagent was used in the wash buffers and in the primary and secondary antibody dilution buffers.

標靶描述

~75 kDa observed. Uniprot describes a molecular weight at ~57 kDa This protein may be observed at ~65 kDa in some cell lysates. (Tamimi, Y., et al. (2006). Hum Mol Genet. 15(21): 3229-3240.).

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
THP cell lysate

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

TWEEN is a registered trademark of Croda International PLC

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable