ABC945

Anti-GMPR Antibody

from rabbit, purified by affinity chromatography

• 同義詞: GMP reductase 1, Guanosine 5′-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: human
• 物種活性（以同源性預測）: primate (based on 100% sequence homology), rat (based on 100% sequence homology), mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• 技術: immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NP_006868
• UniProt登錄號: P36959
• 運輸包裝: wet ice
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GMPR(2766)

描述

一般說明

GMP reductase 1 (EC 1.7.1.7; UniProt P36959; also known as Guanosine 5′-monophosphate oxidoreductase 1, Guanosine monophosphate reductase 1) is encoded by the GMPR (also known as GMPR1) gene (Gene ID 2766) in human. De novo biosynthesis of Guanosine 5′-monophosphate (GMP) is accomplished by reactions catalyzed by several enzymes. Inositol monophosphate (IMP) dehydrogenase 1 and 2 (IMPDH1 and IMPDH2) convert IMP into xanthosine monophosphate (XMP), GMP synthetase (GMPS) then converts XMP into GMP, while guanosine monophosphate reductase (GMPR) catalyzes the reversible conversion of GMP to IMP to fuel back into both the AMP and GMP synthesis pathways. GMPR is shown to reduce intracellular GTP pools, thereby downregulating the level of several GTP-bound (active) RHO-GTPases that are essential for invadopodia formation, extracellular matrix degradation and invasion of melanoma cells both in cultures in vitro and in tumor xenografts in vivo. Consistent with its role as a negative regulator of the invasive melanoma phenotype, GMPR expression is reported to be either undetectable or drastically reduced in human melanoma cell lines when compared to normal melanocytes.

特異性

This polyclonal antibody detects human GMP reductase 1 (GMPR) and stained predominantly the cytoplasm of cells in human tissue sections by immunohistochemistry.

免疫原

Epitope: Between the second and third GMP-binding region.

KLH-conjugated linear peptide corresponding a sequence between the second and third GMP-binding region of human GMPR.

應用

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected GMPR in human prostate cancer, as well as in normal testis and skeletal muscle tissue sections.

Research Category
Apoptosis & Cancer

Research Sub Category
Cytoskeletal Signaling

This Anti-GMPR Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GMPR.

品質

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected GMPR in 10 µg of human skeletal muscle tissue lysate.

標靶描述

~35 kDa observed. 37.42 kDa calculated.

外觀

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

其他說明

Concentration: Please refer to lot specific datasheet.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1