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Merck
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重要文件

ABC432

Sigma-Aldrich

Anti-MAP1LC3B Antibody

rabbit polyclonal

同義詞:

Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related protein LC3 B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 li

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

產品名稱

Anti-LC3B Antibody, serum, from rabbit

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

human

技術

western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

一般說明

MAP1LC3B, also known as Autophagy-related protein LC3 B, LC3B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1 light chain 3-like protein 2, MAP1A/MAP1B light chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-associated protein 1 light chain 3 beta, and encoded by the gene MAP1LC3B/MAP1ALC3, is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). MAP1LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. MAP1LC3B interacts at microtubules with CABP1 (via EF-hands 1 and 2) but not with calmodulin. MAP1LC3B interacts with FYCO1 (via C-terminus). MAP1LC3B interacts with TP53INP1 and TP53INP2, and MAP1LC3B interacts with TBC1D25. MAP1LC3B directly interacts with SQSTM1; this interaction leads to MAP1LC3B recruitment to inclusion bodies containing polyubiquitinated protein aggregates and to inclusion body degradation by autophagy. MAP1LC3B interacts with ATG4B, MAPK15 and BNIP3 protein as well. MAP1LC3B is localized to the cytoplasm and cytoskeleton membranes, specifically endomembranes and cytoplasmic vesicles in the autophagosome. MAP1LC3B is expressed in most abundantly in heart, brain, skeletal muscle and testis tissues, with very low expression in liver tissues. Interestingly, high expression of MAP1LC3B in triple negative breast cancer (TNBC) is associated with poor outcomes in TNBC patients and is currently being studied as a prognostic evaluation marker for patients with TNBC.

免疫原

Linear peptide corresponding to human LC3B.

應用

Detect MAP1 using this rabbit polyclonal antibody, Anti-LC3B Antibody validated for use in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Western Blotting Analysis: A representative lot from an independent laboratory detected LC3B in shRNA control HeLa cell lysates and demonstrated a loss of signal in TBC1D5 knockdown HeLa cell lysates. HeLa cells were either serum starved, or treated with bafilomycin A1 or serum starved in the presence of bafilomycin A1 (Popovic, D., et al. (2012). Mol Cell Biol. 32(9):1733-1744.).
Immunohistochemistry (Paraffin) Analysis: A 1:500 dilution from a representative lot detected LC3B in human cerebral cortex and rat cerebral cortex tissue sections.

品質

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:2,000 dilution of this antibody detected LC3B in 10 µg of HeLa cell lysate.

標靶描述

~15 kDa observed

外觀

Unpurified
Rabbit polyclonal serum with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1


分析證明 (COA)

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Doris Popovic et al.
Molecular and cellular biology, 32(9), 1733-1744 (2012-02-23)
Autophagy is an evolutionarily conserved degradation pathway characterized by dynamic rearrangement of membranes that sequester cytoplasm, protein aggregates, organelles, and pathogens for delivery to the vacuole and lysosome, respectively. The ability of autophagosomal membranes to act selectively toward specific cargo

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