推薦產品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
mouse, human
製造商／商標名
Chemicon®
技術
flow cytometry: suitable
western blot: suitable
輸入
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type: human embryonic stem cell(s)
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
unmodified
基因資訊
human ... NANOG(79923)
mouse ... Nanog(71950)
一般說明
NANOG is a gene expressed in embryonic stem cells (ESCs) and is thought to be a key factor in maintaining pluripotency. NANOG thought to function in concert with other factors such as POU5F1 and SOX2 to establish ESC identity. These cells offer an important area of study because of their ability to maintain pluripotency. In other words, these cells have the ability to become virtually any cell of any of the three germ layers (endoderm, ectoderm, mesoderm).
特異性
Recognizes Nanog, human.
免疫原
Synthetic peptide from human Nanog.
應用
Research Category
Stem Cell Research
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation
Pluripotent & Early Differentiation
This Anti-Nanog Antibody is validated for use in FC, WB for the detection of Nanog.
Western blotting: 1:1,000-1:5,000. The antibody recognizes a protein of ~35 kDa on MBL-5 cell lysate. Additional bands of ~50 and ~60 kDa may be seen depending on sample and antibody dilution used.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
Flow Cytometry: Cormier et al., 2006. Tissue Engineering 12:3233-3245.
Optimal working dilutions must be determined by the end user.
標靶描述
39 kDa (MW 34 kDa)
外觀
Unpurified
Rabbit Serum. Liquid.
儲存和穩定性
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
分析報告
Control
Whole cell lysate from mouse embryonic stem cells
Whole cell lysate from mouse embryonic stem cells
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
A genome-wide RNAi screen identifies a new transcriptional module required for self-renewal.
Hu, G; Kim, J; Xu, Q; Leng, Y; Orkin, SH; Elledge, SJ
Genes & Development null
Guixiang Tan et al.
PloS one, 9(4), e92304-e92304 (2014-04-20)
Activation of signal transducer and activator of transcription 3 (Stat3) by leukemia inhibitory factor (LIF) is required for maintaining self-renewal and pluripotency of mouse embryonic stem cells (mESCs). Here, we have confirmed transcription factor Forkhead Box m1 (Foxm1) as a
Culture conditions and enzymatic passaging of bovine ESC-like cells.
Guochun Gong,Marsha L Roach,Le Jiang,Xiangzhong Yang,Xiuchun Cindy Tian
Cellular Reprogramming null
[Molecular genetic characterization of the regulatory region of the Xist gene in the common vole Microtus rossiaemeridionalis].
K E Orishchenko,E A Elisafenko,A E Kel',S M Zakian
Genetika null
Matthew J Simmons et al.
Breast cancer research : BCR, 14(5), R126-R126 (2012-09-21)
NOTCH activation has been recently implicated in human breast cancers, associated with a poor prognosis, and tumor-initiating cells are hypothesized to mediate resistance to treatment and disease relapse. To address the role of NOTCH1 in mammary gland development, transformation, and
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