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Key Documents

AB9078

Sigma-Aldrich

Anti-Ryanodine Receptor 1 Antibody

serum, Chemicon®

同義詞:

Anti-CCO, Anti-KDS, Anti-MHS, Anti-MHS1, Anti-PPP1R137, Anti-RYDR, Anti-RYR, Anti-RYR-1, Anti-SKRR

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

serum

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

human, mouse, rat

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

human ... RYR1(6261)

特異性

Ryanodine Receptor 1.

免疫原

Synthetic peptide from the variant TM region of human Ryanodine Receptor 1.

應用

Anti-Ryanodine Receptor 1 Antibody detects level of Ryanodine Receptor 1 & has been published & validated for use in IC, IH & WB.
Western blot: 1:1,000 using ECL. The antibody reacts with the 500 kDa Ryanodine Receptor 1 protein. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody), 1% BSA in 0.1M PBS with 0.05% Tween 20. Preferred gel percentage is 4-12% gradient gel.

Immunocytochemistry: 1:1,000

Immunohistochemistry: 1:1,000 overnight at 2-8°C using a fluorescently labeled secondary antibody. Suggested fixative is 4% paraformaldehyde in 0.1M PBS (one hour). Suggested permeablization method is 0.05% Triton X-100 in dilution buffer. Suggested blocking buffer is 10% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS. Suggested dilution buffer is 1% normal goat serum (or same host as your secondary antibody) and 1% BSA in 0.1M PBS.

Optimal working dilutions must be determined by the end user.

分析報告

Control
Western blot = adult mouse skeletal muscle (adult mouse cardiac muscle will be negative).

IHC = adult mouse cerebellum Purkinje cells (adult mouse cerebellum granular cells will be negative).

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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存取文件庫

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Baez-Ruiz, A; Cazares-Gomez, K; Vazquez-Martinez, O; Aguilar-Roblero, R; Diaz-Mu?oz, M
Journal of circadian rhythms null
Skeletal muscle tissue engineering: a maturation model promoting long-term survival of myotubes, structural development of the excitation-contraction coupling apparatus and neonatal myosin heavy chain expression.
Das, M; Rumsey, JW; Bhargava, N; Stancescu, M; Hickman, JJ
Biomaterials null
Immunohistochemical study of microscopic globular bodies of normal human brain.
Saori Odagiri,Fumiaki Mori,Kunikazu Tanji,Naohito Kuroda,Koichi Wakabayashi
Biomedical Research null
Widespread occurrence of eosinophilic neuronal cytoplasmic inclusions in an asymptomatic adult: a novel ubiquitin-negative filamentous inclusion.
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Neuropathology : Official Journal of the Japanese Society of Neuropathology null
Peter Braubach et al.
The Journal of general physiology, 144(5), 393-413 (2014-10-29)
Huntington's disease (HD) is caused by an expanded CAG trinucleotide repeat within the gene encoding the protein huntingtin. The resulting elongated glutamine (poly-Q) sequence of mutant huntingtin (mhtt) affects both central neurons and skeletal muscle. Recent reports suggest that ryanodine

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