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Key Documents

AB5976

Sigma-Aldrich

Anti-Dishevelled-2 Antibody

Chemicon®, from rabbit

同義詞:

DVL2, DSH Homolog 2

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

物種活性

human

製造商/商標名

Chemicon®

技術

western blot: suitable

輸入

sample type hematopoietic stem cell(s)
sample type neural stem cell(s)

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

目標翻譯後修改

unmodified

基因資訊

human ... DVL2(1856)

一般說明

DVL2 is a member of multigene family that also contains DVL1 and DVL3. The vertebrate dishevelled proteins have approximately 40% amino acid sequence similarity with Drosophila dsh. DVL2 encodes a 90-kD protein that undergoes posttranslational phosphorylation to form a 95-kD cytoplasmic protein.

特異性

Dishevelled-2 protein (dvl-2). By Western blot the antibody shows no cross reactivity with recombinant dishevelled-1 (dvl-1) or dishevelled-3 (dvl-3) protein. Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against disheveiled-2 protein.

免疫原

Synthetic peptide corresponding to amino acids 633-645 of human disheveiled-2 protein.

應用

Research Category
Stem Cell Research
Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells
This Anti-Dishevelled-2 Antibody is validated for use in WB for the detection of Dishevelled-2.
Western blot: 1:1,000 using ECL. The antibody reacts with a protein of ~95kDa.

Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

外觀

Format: Purified
Partially purified immunoglobulin. Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

儲存和穩定性

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids


分析證明 (COA)

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