推薦產品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
primate, mouse, rat
製造商／商標名
Chemicon®
技術
immunohistochemistry: suitable
western blot: suitable
UniProt登錄號
運輸包裝
dry ice
目標翻譯後修改
unmodified
基因資訊
human ... TRPV1(7442)
特異性
Capsaicin Receptor (VR1), N-terminal.
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.
SPECIES REACTIVITIES:
Rat, mouse and primate. Other species have not been tested.
免疫原
Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of rat VR1.
應用
Immunoblotting: 1:1,000
Immunohistochemistry: 1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5889
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5889. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOHISTOCHEMISTRY
VR1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
WESTERN BLOT
For details see Guo, A., et al., Eur J Neurosci (1999) 11:946-958.
Immunohistochemistry: 1:1,000
Optimal working dilutions must be determined by the end user.
APPLICATION NOTES FOR AB5889
IMMUNOHISTOCHEMISTRY
Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5889. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope.
IMMUNOHISTOCHEMISTRY
VR1 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above.
WESTERN BLOT
For details see Guo, A., et al., Eur J Neurosci (1999) 11:946-958.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuroinflammation & Pain
Neurotransmitters & Receptors
Neuroinflammation & Pain
Neurotransmitters & Receptors
This Anti-Capsaicin Receptor Antibody, N-terminus is validated for use in WB, IH for the detection of Capsaicin Receptor.
外觀
Liquid. Contains 0.05% sodium azide.
儲存和穩定性
Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer.
法律資訊
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Yannick Marchalant et al.
Neurobiology of disease, 34(2), 300-307 (2009-04-23)
WIN-55,212-2 (WIN-2) can elicit anti-inflammatory and cognitive-enhancing effect in aged rats. The current study was designed to determine the differential role of the endocannabinoid receptor sub-types 1 (CB1) and 2 (CB2) and transient receptor potential vanilloid 1 receptor (TRPV1) in
Min Jee Kim et al.
Molecular pain, 14, 1744806918814345-1744806918814345 (2018-11-02)
The insular cortex is an important region of brain involved in the processing of pain and emotion. Recent studies indicate that lesions in the insular cortex induce pain asymbolia and reverse neuropathic pain. Endogenous cannabinoids (endocannabinoids), which have been shown
TRPV1 stimulation triggers apoptotic cell death of rat cortical neurons.
Hisashi Shirakawa,Tomoko Yamaoka,Kazuaki Sanpei,Hirotoshi Sasaoka,Takayuki Nakagawa,Shuji Kaneko
Biochemical and biophysical research communications null
A Guo et al.
The European journal of neuroscience, 11(3), 946-958 (1999-04-02)
The vanilloid receptor (VR1) protein functions both as a receptor for capsaicin and a transducer of noxious thermal stimuli. To determine the expression and targetting of this protein, we have generated antisera against both the amino and carboxy termini of
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