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Merck
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重要文件

AB5441

Sigma-Aldrich

Anti-Tropomyosin Antibody

Chemicon®, from sheep

同義詞:

Anti-C15orf13, Anti-CMD1Y, Anti-CMH3, Anti-HEL-S-265, Anti-HTM-alpha, Anti-LVNC9, Anti-TMSA

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

sheep

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

mouse, rat

製造商/商標名

Chemicon®

技術

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

特異性

Recognizes Tropomyosin 1, 2, 3, 5a, 5b, 6.

免疫原

Synthetic peptide corresponding to the 9d exon from the alpha gene of tropomyosin.

應用

Research Category
Metabolism
Research Sub Category
Muscle Physiology
This Anti-Tropomyosin Antibody is validated for use in WB, IC, IH for the detection of Tropomyosin.
Western Blot: 1:200-1:1,000. The antibody reacts with the ~29 kDa Tropomyosin protein on mouse brain cytosol. An additional band at ~52-55 kDa may be seen depending on sample and antibody concentration used. It is thought that this band may be a dimer.
Immunocytochemistry: 1:100-1:500
Immunohistochemistry: 1:100-1:500


Optimal working dilutions must be determined by the end user.

外觀

Affinity purified immunoglobulin. Liquid in PBS.

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Tropomyosin localization reveals distinct populations of microfilaments in neurites and growth cones.
Schevzov, G, et al.
Molecular and Cellular Neurosciences, 8, 439-454 (1997)
Anthony J Kee et al.
The Journal of cell biology, 166(5), 685-696 (2004-09-01)
Tropomyosin (Tm) is a key component of the actin cytoskeleton and >40 isoforms have been described in mammals. In addition to the isoforms in the sarcomere, we now report the existence of two nonsarcomeric (NS) isoforms in skeletal muscle. These
Sawako Yamashiro et al.
The Journal of biological chemistry, 289(17), 11616-11629 (2014-03-20)
Tropomodulins (Tmods) are F-actin pointed end capping proteins that interact with tropomyosins (TMs) and cap TM-coated filaments with higher affinity than TM-free filaments. Here, we tested whether differences in recognition of TM or actin isoforms by Tmod1 and Tmod3 contribute
Eui-Jung Park et al.
American journal of physiology. Renal physiology, 304(7), F958-F971 (2013-01-11)
It has been reported that several proteins [heat shock protein 70 (Hsp70 and Hsc70), annexin II, and tropomyosin 5b] interact with the Ser(256) residue on the COOH terminus of aquaporin-2 (AQP2), where vasopressin-induced phosphorylation occurs for mediating AQP2 trafficking. However
Functional effects of mutations in the tropomyosin-binding sites of tropomodulin1 and tropomodulin3.
Lewis, RA; Yamashiro, S; Gokhin, DS; Fowler, VM
Cytoskeleton (Hoboken, N.J.) null

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