AB5382

Anti-Nitric Oxide Synthase II Antibody

serum, Chemicon^®

• 同義詞: NOS II, iNOS
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: serum
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 物種活性: mouse, rat
• 製造商／商標名: Chemicon^®
• 技術: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI登錄號: NM_153292.1
• UniProt登錄號: P35228
• 運輸包裝: dry ice
• 目標翻譯後修改: unmodified
• 基因資訊: mouse ... Nos2(18126) ; rat ... Nos2(24599)

描述

一般說明

Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.

特異性

Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry.

Reactivity to human iNOS is approximately 1%.

免疫原

C-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase.

Epitope: C-terminus

應用

Anti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB.

Immunohistochemistry:
1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver).

Immunocytochemistry:
1:50,000 dilution of a previous lot was used on cultured cells.

Western blot:
1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

品質

Evaluated by Western Blot on Mouse liver lysates.

Western Blot Analysis:
1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates.

標靶描述

131 kDa

外觀

Unpurified

Rabbit polyclonal serum in buffer containing liquid 0.05% sodium azide.

儲存和穩定性

Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析報告

Control
Macrophages, liver lysates

其他說明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1