AB4332

Anti-acetyl-MVH (Lys405) Antibody

from rabbit, purified by affinity chromatography

• 同義詞: Probable ATP-dependent RNA helicase DDX4, DEAD box protein 4, Mvh, Vasa homolog
• 分類程式碼代碼: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

屬性

• 生物源: rabbit
• 品質等級: 100
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 純化經由: affinity chromatography
• 物種活性: pig, human, mouse
• 物種活性（以同源性預測）: porcine (based on 100% sequence homology), rat (based on 100% sequence homology)
• 技術: dot blot: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable (peptide); western blot: suitable
• NCBI登錄號: NP_034159
• UniProt登錄號: Q61496
• 運輸包裝: wet ice
• 目標翻譯後修改: acetylation (Lys405)
• 基因資訊: mouse ... Ddx4(13206)

描述

一般說明

MVH (also known as DDX4 or Vasa) is a member of the DEAD family of proteins and is a probable ATP-dependent RNA helicase. MVH is mostly localized in testicular germ cells in the cytoplasm of spermatocytes. MVH is a high-copy extragenic suppressor gene. MVH is shown to be significantly decreased in spermatozoa of men that are infertile and may have an important role in male fertility and in stem cell development.

特異性

This antibody specifically recognizes wild type MVH acetylated at Lys405, but not the Lys405 mutant.

免疫原

Epitope: Acetylated Lys405

KLH-conjugated linear peptide corresponding to mouse MVH acetylated at Lys405.

應用

Anti-acetyl-MVH (Lys405) Antibody is a Rabbit Polyclonal Antibody for detection of acetyl-MVH (Lys405) also known as Probable ATP-dependent RNA helicase DDX4, DEAD box protein & has been validated in DB, WB, ICC, PIA & IP.

Research Category
Stem Cell Research

Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Western Blot/Immunoprecipitation Analysis: A representative lot detected Flag-tagged acetyl-MVH (Lys405) in the presence of Myc-HAT/p46.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected acetyl-MVH (Lys405) in mouse germ cells (I. Nagamori, Department of Pharmacology, University of California, Irvine).

品質

Evaluated by Dot Blot/Peptide Inhibition Assay in non-acetylated and acetylated MVH (Lys405).

Dot Blot/Peptide Inhibition Analysis: A 1:1,000 dilution of this antibody detected acetyl-MVH (Lys405) peptide.

標靶描述

76 kDa calculated

外觀

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

儲存和穩定性

Stable for 1 year at 2-8°C from date of receipt.

分析報告

Control
Non-acetylated and acetylated MVH (Lys405)

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids