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Key Documents

AB1632

Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

Chemicon®, from rabbit

同義詞:

NOS I, bNOS, nNOS

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About This Item

分類程式碼代碼:
12352203
eCl@ss:
32160702
NACRES:
NA.41

生物源

rabbit

品質等級

抗體表格

affinity purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

polyclonal

純化經由

affinity chromatography

物種活性

bovine, mouse, rat

製造商/商標名

Chemicon®

技術

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

NCBI登錄號

UniProt登錄號

運輸包裝

dry ice

目標翻譯後修改

unmodified

基因資訊

mouse ... Nos1(18125)

特異性

Reactive with human brain NOS-I (nNOS). Antibody binding is neutralized in Western blot by pre-adsorption with the immunogen peptide. This sequence is highly conserved in human, rat and mouse.

免疫原

Synthetic peptide corresponding to amino acids 724-739 of human brain NOS, coupled to KLH.

應用

Anti-Nitric Oxide Synthase I Antibody is an antibody against Nitric Oxide Synthase I for use in ELISA, WB, IH.
Immunohistochemistry: Use at 5 - 10 μg/mL on rat neuronal sections fixed with 3.5% paraformaldehyde. Incubation overnight at 4°C with a 1:100 - 1:500 dilution. ABC (peroxidase) detection. Positive staining of Purkinje cells and Layer 5 primary neurons.

Western blot: 1 - 10 μg/mL with chemiluminescent detection. Higher concentrations of the antibody may be necessary if colorimetric detection is used. Antibody dilution will also vary with the concentration of antigen. Then antibody should recognize a 155 kDa protein.

ELISA: Use at 1.0 μg/mL or lower on plates coated with 50 - 100 ng of peptide antigen.

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Oxidative Stress

外觀

Peptide affinity-purified immunoglobulin. Supplied at a 1mg/mL solution in PBS, pH 7.4, containing 0.1% BSA.

儲存和穩定性

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated thawing and refreezing.

法律資訊

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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Lateralization of hippocampal nitric oxide mediator system in people with Alzheimer disease, multi-infarct dementia and schizophrenia.
Zdena Kristofikova,Iryna Kozmikova,Petra Hovorkova,Jan Ricny,Petr Zach,Emmerich Majer et al.
Neurochemistry International null
Rouba Ali-Fehmi et al.
Cancer, 117(2), 301-309 (2010-09-08)
Ovarian epithelial carcinomas have recently been classified as slow growing type I tumors and rapidly growing highly aggressive type II tumors. The present study sought to molecularly characterize type I and II tumors using known molecular markers. Specimens from 213
Structural, histochemical and immunocytochemical study of the forestomach mucosa in domestic ruminants.
Scala G, Corona M, Maruccio L
Anatomia, Histologia, Embryologia null
D S Bredt et al.
Nature, 351(6329), 714-718 (1991-06-27)
Nitric oxide is a messenger molecule, mediating the effect of endothelium-derived relaxing factor in blood vessels and the cytotoxic actions of macrophages, and playing a part in neuronal communication in the brain. Cloning of a complementary DNA for brain nitric
A Özen et al.
Anatomia, histologia, embryologia, 42(5), 384-393 (2013-02-16)
This study was aimed to determine staining intensity, cellular localization and distribution of the nitric oxide synthase (NOS) enzymes during the sexual cycle in the cow oviduct. Oviduct samples belonging to 20 cows, 10 of which were in the estrual

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