推薦產品
生物源
rabbit
品質等級
抗體表格
serum
抗體產品種類
primary antibodies
無性繁殖
polyclonal
物種活性
mouse, human
物種活性（以同源性預測）
rat (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登錄號
UniProt登錄號
運輸包裝
wet ice
目標翻譯後修改
ubiquitination (Lys119)
基因資訊
bovine ... H2Ax(531733)
dog ... H2Ax(489372)
human ... H2AX(3014)
mouse ... H2Ax(15270)
rat ... H2Ax(500987)
一般說明
H2AX is involved in DNA repair and the maintenance of genomic stability. It is been implicated both in homologous recombination and nonhomologous end joining DNA repair pathways. H2AX is thought to have a critical function in the recruitment of DNA repair factors and DNA damage-signaling proteins. H2A.X is ubiquitinated by RING1 and RNF2/RING2 complex providing a specific mark for transcriptional repression. In addition this protein can be ubiquitinated by the E2 ligase UBE2N and the E3 ligases RNF8 and RNF168 as result of double-stranded DNA breaks to recruit repair proteins a damaged site. H2AX is phosphorylated soon after the appearance of double-stranded breaks. Hyperphoshorylation of H2AX may be linked to chromatin fragmentation prior to apoptosis.
特異性
This antibody recognizes Histone H2A.X when ubiquitylated at Lys119.
免疫原
Epitope: Lys119
Linear peptide containing ubiquitylated Lys119 of human Histone H2A.X.
應用
Anti-Ubiquityl Histone H2A.X (Lys119) Antibody is a rabbit polyclonal antibody for detection of Ubiquityl Histone H2A.X (Lys119) also known as H2AXK119Ub, Histone H2A.X Ubiquityl K119) & has been validated in WB, IP, ICC.
Immunocytochemsitry Analysis: 1:500 dilution from a representative lot detected Histone H2A.X in RAW264.7 and NIH/3T3 cells.
Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
Immunoprecipitation Analysis: 5 µl from a representative lot immunoprecipitated Histone H2A.X in HeLa acid extract.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Histones
Histones
品質
Evaluated by Western Blot in HeLa acid extract.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.
Western Blot Analysis: 1:1,000 dilution of this antibody detected Histone H2A.X on 10 µg of HeLa acid extract.
標靶描述
~ 23 kDa
外觀
Unpurified
Unpurified rabbit polyclonal containing serum with 0.05% sodium azide.
儲存和穩定性
Stable for 1 year at -20°C from date of receipt.
分析報告
Control
HeLa acid extract
HeLa acid extract
其他說明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 1
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
Stéphanie Olivier-Van Stichelen et al.
Frontiers in genetics, 5, 256-256 (2014-08-20)
O-GlcNAc Transferase (OGT) catalyzes protein O-GlcNAcylation, an abundant and dynamic nuclear and cytosolic modification linked to epigenetic regulation of gene expression. The steady-state levels of O-GlcNAc are influenced by extracellular glucose concentrations suggesting that O-GlcNAcylation may serve as a metabolic
Yannick D Benoit et al.
Journal of cellular physiology, 228(4), 764-772 (2012-09-25)
Colorectal cancer is ranked among the top leading causes of cancer death in industrialized populations. Polycomb group proteins, including Suz12 and Ezh2, are epigenetic regulatory proteins that act as transcriptional repressors of many differentiation-associated genes and are overexpressed in a
Subhasree Roy Choudhury et al.
Carcinogenesis, 32(10), 1525-1532 (2011-07-30)
Polycomb group (PcG) protein-dependent histone methylation and ubiquitination drives chromatin compaction leading to reduced tumor suppressor expression and increased cancer cell survival. Green tea polyphenols and S-adenosylhomocysteine (AdoHcy) hydrolase inhibitors are important candidate chemopreventive agents. Previous studies indicate that (-)-epigallocatechin-3-gallate
Sivaprakasam Balasubramanian et al.
Cellular signalling, 27(7), 1336-1344 (2015-04-07)
The Bmi-1 Polycomb group (PcG) protein is an important epigenetic regulator of chromatin status. Elevated Bmi-1 expression is observed in skin cancer and contributes to cancer cell survival. (-) Epigallocatechin-3-gallate (EGCG), an important green tea-derived cancer prevention agent, reduces Bmi-1
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