70045-M

pBAC4x-1 DNA - Novagen

• 分類程式碼代碼: 41106612
• NACRES: NA.51

屬性

• 形狀: liquid
• 品質等級: 100
• 製造商／商標名: Novagen^®
• 儲存條件: OK to freeze; avoid repeated freeze/thaw cycles
• 儲存溫度: −20°C

描述

一般說明

pBAC4x-1 is a baculovirus transfer plasmid designed for cloning and co-expression of up to four target genes or multiple copies of the same gene in insect cells. The plasmid contains two polh promoters and two p10 promoters, each of which is upstream of unique cloning sites for sequential insertion of target genes. The homologous promoters are in opposite orientations to minimize recombination. The plasmid is compatible with BacVector-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the promoters. The vector provides optional expression of a C-terminal His•Tag fusion sequence from the polh right-hand promoter by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the clockwise promoters is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to this strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.

警告

Toxicity: Standard Handling (A)

法律資訊

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable