推薦產品
生物源
mouse
品質等級
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
TAU-5, monoclonal
形狀
liquid
不包含
preservative
物種活性
mouse, human, sheep, rat
製造商/商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
同型
IgG1
運輸包裝
wet ice
儲存溫度
−70°C
目標翻譯後修改
unmodified
基因資訊
human ... MAPT(4137)
mouse ... Mapt(17762)
rat ... Mapt(29477)
一般說明
蛋白G纯化的小鼠单克隆抗体。识别约45-68 kDa磷酸化和非磷酸化形式的tau。
识别~45-68 kDa Tau蛋白。
该小鼠抗Tau mAb(TAU-5)经过验证,可用于冷冻切片、免疫印迹、免疫沉淀、石蜡切片以检测Tau。
免疫原
牛
纯化的牛微管相关蛋白
表位:中心区域内
應用
冷冻切片(1-2 µg/ml)
免疫印迹(1 µg/ml)
免疫沉淀(每200-500 µg细胞裂解液 10 µg)
石蜡切片(1-2 µg/ml,需要热预处理)
免疫印迹(1 µg/ml)
免疫沉淀(每200-500 µg细胞裂解液 10 µg)
石蜡切片(1-2 µg/ml,需要热预处理)
包裝
特定浓度批号请参考小瓶的标签。
警告
毒性:标准处理(A)
外觀
在PBS中。
重構
初次解冻后,分装冻存(-70°C)。如果浓度为<50 µg/ml,请勿贮藏没有载体蛋白的稀释抗体。
其他說明
Papasozomenos, S.C. and Shanavas, A., 2002.Proc.Natl.Acad.Sci. USA99, 1140.
Rapoport, M., eta al.2002.Proc.Natl.Acad.Sci. USA99, 6364.
Rapoport, M., eta al.2002.Proc.Natl.Acad.Sci. USA99, 6364.
当用于福尔马林/石蜡包埋切片时,在10 mM枸橼酸盐缓冲液(pH 6.0)中煮沸组织切片10-20分钟,然后在抗体培养前在室温下冷却20分钟,从而增强染色。Tau mRNA的交替剪接和差异磷酸化促成tau的异质性。不与微管蛋白的其他MAPS发生交叉反应。识别天然和磷酸化形式的tau。合适的工作稀释度由与测定条件相关的变量决定。
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類(WGK)
WGK 1
閃點(°F)
Not applicable
閃點(°C)
Not applicable
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號,寫有 ‘Lot’或‘Batch’.。
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