516483

Pepstatin A, Penetratin

A Pepstatin A that is ~75% less potent against Cathespins D/E than PepA in cell-free assays.

• 同義詞: Pepstatin A, Penetratin, PepA-RQIKIWFQNRRMKWKK-OH, PepA-Antp₄₃₋₅₈
• 經驗公式（希爾表示法）: C₁₃₈H₂₂₉N₃₉O₂₈S
• 分子量：: 2914.60
• 分類程式碼代碼: 12352202
• NACRES: NA.77

屬性

• 品質等級: 100
• 化驗: ≥97% (HPLC)
• 形狀: lyophilized solid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 顏色: white
• 溶解度: water: 1 mg/mL
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

A Pepstatin A (PepA; Cat. No. 516481) penetratin (pAntp_43-58) conjugate (PepA-P) that is ~75% less potent against Cathespins D/E (IC₅₀ = 920 nM) than PepA in cell-free assays, presumably due to its decreased solubility. However, with its enhanced permeability, PepA-P is much more active than PepA in inhibiting cellular aspartic protease activity in MCF7 (94.1% vs. 54.6% inhibition wtih 10 µM respective compound), Boleths, and Dendritic cultures. PepA-P, but not PepA, effectively interferes with antigen processing by primary APCs and the subsequent activation of antigen-specific memory T cells.

包裝

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

序列

PepA-Arg-Gln-Ile-Lys-Ile-Trp-Phe-Gln-Asn-Arg-Arg-Met-Lys-Trp-Lys-Lys-CO₂H

外觀

Supplied as a trifluoroacetate salt.

重構

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

其他說明

Zaidi, N., et al. 2007. Biochem. Biophys. Res. Commun.364, 243.

法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable