495609

Okadaic Acid, Prorocentrum sp.

InSolution, ≥95%, inhibitor of protein phosphatase 1

• 同義詞: InSolution Okadaic Acid, Prorocentrum sp.
• 經驗公式（希爾表示法）: C₄₄H₆₈O₁₃
• 分子量：: 805.00
• 分類程式碼代碼: 12352106

屬性

• 品質等級: 100
• 化驗: ≥95% (HPLC)
• 形狀: liquid
• 製造商／商標名: Calbiochem^®
• 儲存條件: OK to freeze; desiccated (hygroscopic); protect from light
• 運輸包裝: wet ice
• 儲存溫度: −20°C

描述

一般說明

An ionophore-like polyether derivative of a C₃₈ fatty acid compound isolated from dinoflagellates that have fed on the marine sponge Halinchrondria okadai. It is a potent non-comepetitive, reversible inhibitor of serine/threonine-specific protein phosphatases 1 (PP1, IC₅₀ = 10-15 nM, rabbit skeletal muscle, catalytic subunit) and 2A (PP2A, IC₅₀ = 0.1 nM, rabbit skeletal muscle, catalytic subunit). It has only a trivial effect on protein phosphatase 2B (IC₅₀ = 5 µM), a Ca²⁺/calmodulin-dependent enzyme, while PP2C, a Mg²⁺ dependent enzyme, is unaffected. Okadaic acid has no significant effect on the activities of tyrosine phosphatases, alkaline phosphatase, acid phosphatase, and inositol trisphosphatase. It is also a non-phorbol ester-type tumor promoter on mouse skin. Useful for the study of protein phosphatases and of PP1/PP2A in cell extracts, as well as in intact cells. Induces apoptosis in human breast carcinoma (MB-231 and MCF-7) and in myeloid cells, but inhibits glucocorticoid-induced apoptosis in T-cell hybridomas. Has marked contractile effect on smooth muscles and heart muscles. Implicated as a causative agent of diarrhetic shellfish poisoning.

生化／生理作用

Cell permeable: no

Primary Target
protein phosphatase

Product does not compete with ATP.

Reversible: no

Target IC₅₀: 10-15 nM against protein phosphatase 1; 0.1 nM against protein phosphatase 2A

包裝

Packaged under inert gas

警告

Toxicity: Toxic (F)

外觀

A 250 µM (25 µg/124 µl) solution of Okadaic Acid, (Cat. No. 495604) in DMSO.

重構

Following initial thaw, aliquot and freeze (-20°C).

其他說明

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

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法律資訊

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: 188.6 °F - closed cup - (Dimethylsulfoxide)
• 閃點（°C）: 87 °C - closed cup - (Dimethylsulfoxide)